Tumor angiogenesis is a complex process that is unamenable to intravital whole-process monitoring, especially on microscopic assessment of tumor microvessel and quantifying microvascular hemodynamics before and after the nanotherapeutics, which hinder the understanding of nanotheranostics outcomes in tumor treatment. Herein, a new photoacoustic (PA) imaging-optical coherence tomography angiography (OCTA)-laser speckle (LS) multimodal imaging strategy is first proposed, which is not only able to precisely macro guide the thermo-chemotherapy of tumor by monitoring blood oxygen saturation (SaO₂) and hemoglobin content (HbT), but also capable of long-term microscopic investigating the microvessel morphology (microvascular density) and hemodynamics changes (relative blood flow) before and after the nanotherapeutics in vivo. Moreover, to realize the tumor thermo-chemotherapy treatment based on this novel multimodal imaging strategy, a 2D 5-fluorouracil silicon nanosheets (5-Fu-Si NSs) therapeutic agent is designed. Furthermore, 2D high-resolution tumor microvascular images in different stage display that tendency of the thermo-chemotherapy effect is closely associated with tumor angiogenesis. Taken together, the investigations establish the fundamental base in theory and technology for further tailoring the novel specific diagnosis and treatment strategy in tumor. More importantly, this technique will be beneficial to evaluate the tumor microvascular response to nanotherapeutics at microscale.

中文翻译：

---

通过二维硅纳米平台进行活体全程监测热化疗：宏观指导和长期显微精确成像策略

肿瘤血管生成是一个复杂的过程，难以进行活体全过程监测，特别是在纳米治疗前后对肿瘤微血管的微观评估和微血管血流动力学的量化，这阻碍了对肿瘤治疗中纳米治疗结果的理解。在此，首次提出了一种新的光声（PA）成像-光学相干断层扫描血管造影（OCTA）-激光散斑（LS）多模态成像策略，不仅能够通过监测血氧饱和度来宏观精确指导肿瘤的热化疗(SaO ₂ )和血红蛋白含量(HbT)，还能够长期显微研究体内纳米治疗前后的微血管形态(微血管密度)和血流动力学变化(相对血流量)。此外，为了实现基于这种新型多模态成像策略的肿瘤热化疗治疗，设计了一种2D 5-氟尿嘧啶硅纳米片（5-Fu-Si NSs）治疗剂。此外，不同阶段的二维高分辨率肿瘤微血管图像显示，热化疗效果的趋势与肿瘤血管生成密切相关。总而言之，这些研究为进一步制定肿瘤特异性新型诊断和治疗策略奠定了理论和技术基础。更重要的是，该技术将有利于在微观尺度上评估肿瘤微血管对纳米治疗药物的反应。