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USP38 Couples Histone Ubiquitination and Methylation via KDM5B to Resolve Inflammation
Advanced Science ( IF 14.3 ) Pub Date : 2021-06-24 , DOI: 10.1002/advs.202101964
Zhiyao Zhao , Zexiong Su , Puping Liang , Di Liu , Shuai Yang , Yaoxing Wu , Ling Ma , Junyan Feng , Xiya Zhang , Chenglei Wu , Junjiu Huang , Jun Cui

Adv. Sci. 2020, 7, 2002680

DOI: 10.1002/advs.202002680

In the originally published article, the same figure for Figure S3 A and B were shown (Supporting Information). Please find the correct Figure S3 here:

image
Figure S3
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USP38 is dispensable for the TLR-stimulated signaling cascade. (A-B) Immunoblot analysis of phosphorylated (p-) and total proteins in whole-cell lysates of WT and Usp38-KO BMDMs (A) and BMDCs (B) that were stimulated with LPS for the indicated time points. (C) Immunoblot analysis of phosphorylated (p-) and total protein in whole-cell lysates of USP38-inducible THP1 cells with or without DOX that were stimulated with LPS for the indicated time points. (D) Immunoblot analysis of phosphorylated p65 in PBMCs silenced with control siRNA (siCtrl) or USP38-specific siRNA (siUSP38) under LPS treatment for the indicated time points. (E) Immunoblot analysis of USP38 in the chromatin-bound and unbound components of WT and Usp38-KO BMDMs under LPS treatment for the indicated time points. Data are representative of 3 independent biological experiments.

The authors apologize for any inconvenience this may have caused.



中文翻译:


USP38 通过 KDM5B 耦合组蛋白泛素化和甲基化来解决炎症



副词。科学2020年7月2002680


DOI:10.1002/advs.202002680


在最初发表的文章中,显示了图 S3 A 和 B 的相同图(支持信息)。请在此处找到正确的图 S3:

image
 图S3

在图查看器PowerPoint中打开

USP38 对于 TLR 刺激的信号级联是可有可无的。 (AB) 对用 LPS 刺激指定时间点的 WT 和 Usp38-KO BMDM (A) 和 BMDC (B) 的全细胞裂解物中的磷酸化 (p-) 和总蛋白进行免疫印迹分析。 (C) 使用或不使用 DOX 的 USP38 诱导型 THP1 细胞的全细胞裂解物中磷酸化 (p-) 和总蛋白的免疫印迹分析,这些细胞用 LPS 刺激指定的时间点。 (D) 在 LPS 处理指定时间点下,用对照 siRNA (siCtrl) 或 USP38 特异性 siRNA (siUSP38) 沉默的 PBMC 中磷酸化 p65 的免疫印迹分析。 (E) 在 LPS 处理指定时间点下,对 WT 和 Usp38-KO BMDM 的染色质结合和未结合成分中的 USP38 进行免疫印迹分析。数据代表 3 个独立的生物实验。


作者对由此造成的任何不便表示歉意。

更新日期:2021-06-24
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