Synthesis of cationic carbon dots and their effects on human serum proteins and in vitro blood coagulation,Luminescence

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Synthesis of cationic carbon dots and their effects on human serum proteins and in vitro blood coagulation
Luminescence ( IF 3.2 ) Pub Date : 2021-06-23 , DOI: 10.1002/bio.4108
Hua Qiao ₁ , Huichao Zhang ₁ , Ajab Khan ₁ , Bo Li ₁ , Lin Ge ₂ , Yequn Liu ₂ , Wei Bian ₁ , Zhizhen Liu ₁ , Jun Xie ₁

Affiliation

Cationic carbon dots (CCDs) are a promising alternative to gene-delivery systems, and good biosafety levels are crucial for their in vivo use. In this study, spherical and monodispersed CCDs with an average surface potential of +28.7 mV were prepared using sucrose and glutamate (denoted SG-CCDs) using a one-pot autoclave-assisted method. Molecular interactions between the SG-CCDs and four major human serum proteins (albumin, immunoglobulin G, fibrinogen, and transferrin) were investigated. The results were further verified on human serum, and the effect of the SG-CCDs on in vitro blood coagulation was examined. The results showed that the fluorescence of human serum was clearly quenched by the SG-CCDs through a dynamic collision mechanism. Moreover, SG-CCDs at a concentration of 20 μM exhibited minor effects on the secondary structure of human serum. The activated partial thromboplastin and prothrombin time as well as the fibrinogen concentration were not changed, indicating that the SG-CCDs did not interfere with the coagulation process. This study provided an understandable background on the behaviour of CCDs in clinical applications.

中文翻译：

阳离子碳点的合成及其对人血清蛋白和体外凝血的影响

阳离子碳点 (CCD) 是基因传递系统的有前途的替代品，良好的生物安全水平对其体内使用至关重要。在这项研究中，使用一锅高压釜辅助方法，使用蔗糖和谷氨酸（表示为 SG-CCD）制备了平均表面电位为 +28.7 mV 的球形和单分散 CCD。研究了 SG-CCD 和四种主要人血清蛋白（白蛋白、免疫球蛋白 G、纤维蛋白原和转铁蛋白）之间的分子相互作用。结果在人血清上得到了进一步验证，SG-CCDs 对体外的影响检查了血液凝固。结果表明，SG-CCDs通过动态碰撞机制清楚地淬灭了人血清的荧光。此外，浓度为 20 μM 的 SG-CCD 对人血清的二级结构影响较小。活化的部分凝血活酶和凝血酶原时间以及纤维蛋白原浓度没有变化，表明 SG-CCDs 没有干扰凝血过程。这项研究为 CCD 在临床应用中的行为提供了一个可以理解的背景。

更新日期：2021-06-23

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