Neuroblastoma (NB) is an estrogen-dependent tumor. Mono-2-ethylhexyl phthalate (MEHP) has an estrogen-like effect. However, the effects of MEHP on the progression of NB are not well illustrated. This study was to clarify the effect of Notch pathway on proliferation and cell cycle of SH-SY5Y cell induced by MEHP. The viability of SH-SY5Y and BE2C cells were detected by CCK8; cell cycle and apoptosis were detected by flow cytometry; the protein expression levels of Notch pathway and cell cycle related proteins were detected by Western-blot. Results show that MEHP exposure can promote cell proliferation and altered the cell cycle. MEHP exposure can up-regulate the expression of C-MYC, Cyclin D1, Bcl-2 and affected the Notch pathway. In conclusion, MEHP exposure can promote NB cell proliferation and affect the cell cycle and apoptosis. Notch pathway plays a critical role in accelerating the cell cycle and inhibiting the apoptosis of SH-SY5Y cells caused by MEHP.

中文翻译：

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Notch通路在邻苯二甲酸单2-乙基己酯对SH-SY5Y细胞增殖和细胞周期影响中的作用

神经母细胞瘤（NB）是一种雌激素依赖性肿瘤。邻苯二甲酸单-2-乙基己酯 (MEHP) 具有雌激素样作用。然而，MEHP 对 NB 进展的影响并未得到很好的说明。本研究旨在阐明Notch通路对MEHP诱导的SH-SY5Y细胞增殖和细胞周期的影响。通过CCK8检测SH-SY5Y和BE2C细胞的活力；流式细胞术检测细胞周期和凋亡；通过Western-blot检测Notch通路和细胞周期相关蛋白的蛋白表达水平。结果表明，MEHP 暴露可促进细胞增殖并改变细胞周期。MEHP暴露可上调C-MYC、Cyclin D1、Bcl-2的表达并影响Notch通路。综上所述，MEHP暴露可促进NB细胞增殖并影响细胞周期和凋亡。