ABSTRACT

In recent years, there has been an apparent Europe-wide emerging presence of brown spot needle blight, a disease of pine species caused by the fungus Lecanosticta acicola. In this study, we report the first well-documented occurrences of the pathogen in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region and TEF1 gene. Among the material collected from 84 locations within the country, L. acicola was present in 17 samples from 13 different locations, mainly distributed in urban environments. Four pine species were identified as hosts, among which, Pinus nigra and P. mugo were found to be the most frequently infected. Analysis of the mating type genes of 24 isolates obtained from two localities revealed the presence of a single mating type in Slovakia. All analyzed ITS sequences of the Slovak isolates were found to be uniform. However, although analysis of the TEF1 gene indicated that all Slovak isolates could be grouped into a single lineage, we detected nucleotide polymorphisms suggestive of a certain degree of genetic diversification within central European populations of the fungus.

摘要

近年来，欧洲范围内明显出现了褐斑针叶枯病，这是一种由Lecanosticta acicola真菌引起的松树种病害。在这项研究中，我们报告了斯洛伐克第一次有据可查的病原体，使用物种特异性引物进行分子鉴定，并基于 ITS 区域和TEF1基因的测序。在从全国 84 个地点收集的材料中，L. acicola存在于来自 13 个不同地点的 17 个样本中，主要分布在城市环境中。四种松树被确定为宿主，其中黑松和P. mugo被发现是最常感染的。对从两个地方获得的 24 种分离株的交配型基因进行分析，揭示了在斯洛伐克存在单一交配型。发现所有分析的斯洛伐克分离株的 ITS 序列都是一致的。然而，尽管对TEF1基因的分析表明所有斯洛伐克分离株都可以归为一个谱系，但我们检测到核苷酸多态性，表明该真菌的中欧种群存在一定程度的遗传多样性。