The black koji mold, Aspergillus luchuensis, which belongs to Aspergillus section Nigri, is used for the production of traditional Japanese spirits (shochu) mainly in the southern districts of Japan. This mold is known to produce amylolytic enzymes essential for shochu production; however, mechanisms regulating amylolytic gene expression in A. luchuensis have not been studied in as much detail as those in the yellow koji mold, Aspergillus oryzae. Here, we examined the gene expression profiles of deletion mutants of transcription factors orthologous to A. oryzae AmyR and CreA in A. luchuensis. A. luchuensis produces acid-unstable (AmyA) and acid-stable (AsaA) α-amylases. AmyA production and amyA gene expression were not influenced by amyR or creA deletion, indicating that amyA was constitutively expressed. In contrast, asaA gene expression was significantly down- and upregulated upon deletion of amyR and creA, respectively. Furthermore, the glaA and agdA genes (encoding glucoamylase and α-glucosidase, respectively) showed expression profiles similar to those of asaA. Thus, genes that play pivotal roles in starch saccharification, asaA, glaA, and agdA, were found to be regulated by AmyR and CreA. Moreover, despite previous reports on AsaA being only produced in solid-state culture, deletion of the ortholog of A. oryzae flbC, which is involved in the expression of the solid-state culture-specific genes, did not affect AsaA α-amylase activity, suggesting that FlbC was not associated with asaA expression.

黑曲霉菌，曲霉属琉球，属于曲霉属部Nigri，被用于生产传统的日本烈酒（的烧酒）主要在日本南部地区。众所周知，这种霉菌会产生烧酒生产所必需的淀粉分解酶；然而，在调节淀粉分解的基因表达的机制A.琉球没有被研究尽量详细那些在黄曲霉菌，米曲霉。在这里，我们检查了与A. oryzae AmyR 和 CreA直系同源的转录因子的缺失突变体的基因表达谱。A. luchuensis . A. luchuensis产生酸不稳定 (AmyA) 和酸稳定 (AsaA) α-淀粉酶。AmyA 的产生和amyA基因的表达不受amyR或creA缺失的影响，表明amyA是组成型表达的。相比之下，asaA基因表达分别在amyR和creA缺失后显着下调和上调。此外，glaA和agdA基因（分别编码葡糖淀粉酶和α-葡糖苷酶）显示出与asaA相似的表达谱. 因此，发现在淀粉糖化中起关键作用的基因asaA、glaA和agdA受 AmyR 和 CreA 调节。此外，尽管之前有报道称 AsaA 仅在固态培养中产生，但参与固态培养特异性基因表达的米曲霉 flbC直系同源基因的缺失并未影响 AsaA α-淀粉酶活性，表明 FlbC 与asaA表达无关。