ABSTRACT

Green shield bug (GSB) Palomena prasina (L.) causes significant yield and quality losses in hazelnut production of Turkey. Alive and dead adults of GSB were collected from the main hazelnut cultivation areas of Turkey during 2018 and 2019. The entomopathogenic fungi (EPF) were isolated from GSB individuals and identified by DNA sequencing of the internal transcribed spacer of the rRNA. Phylogenetic analysis based on the maximum likelihood method of these sequences with reference sequences retrieved from GenBank revealed that 20 native isolates were Beauveria bassiana (6), B. pseudobassiana (1), Cordyceps confragosa (5), Akanthomyces muscarius (4), Purpureocillium lilacinum (2), Isaria fumosorosea (1), and Bionectria sp. (1). Furthermore, the isolates were tested for their efficacy against GSB adults at 1 × 10⁸ spores mL⁻¹ concentration under laboratory conditions. Registered bio-insecticides were applied at the same concentration to compare the efficacy of the isolates. Beauveria spp. isolates were most effective among the tested isolates with LT₅₀ and LT₉₀ values ranging between 3.65–6.14 and 5.26–8.25 days, respectively. These isolates caused 100% mortality in GSB adults within 6–10 days. The isolates of A. muscarius (TR-OR-1) and P. lilacinum (TR-SM-7) were highly effective and caused 100% mortality 10 and 11 days after the treatment, respectively. These results indicated that some EPF isolates obtained in this study are more promising as an alternative control agent against GSB.

摘要

绿盾虫 (GSB) Palomena prasina (L.) 在土耳其榛子生产中造成显着的产量和质量损失。2018 年和 2019 年期间，从土耳其主要榛子种植区收集了 GSB 的活和死成虫。 从 GSB 个体中分离出昆虫病原真菌 (EPF)，并通过 rRNA 的内部转录间隔区的 DNA 测序进行鉴定。基于与从GenBank中检索到的参考序列的这些序列的最大似然法系统发育分析表明，20个天然分离株球孢白僵菌（6），B. pseudobassiana（1），冬虫夏草confragosa（5），Akanthomyces muscarius（4），Purpureocillium lilacinum (2),Isaria fumosorosea (1) 和Bionectria sp。(1) 。此外，在实验室条件下以1 × 10 ⁸孢子mL ^-1浓度测试分离株对GSB成虫的效力。以相同浓度施用已注册的生物杀虫剂以比较分离株的功效。白僵菌属。LT ₅₀和 LT ₉₀值分别在 3.65-6.14 天和 5.26-8.25 天之间的测试分离株中最有效。这些分离株在 6-10 天内导致 GSB 成人 100% 的死亡率。A. muscarius (TR-OR-1) 和P. lilacinum的分离物(TR-SM-7) 非常有效，分别在治疗后 10 天和 11 天导致 100% 的死亡率。这些结果表明，本研究中获得的一些 EPF 分离株更有希望作为 GSB 的替代控制剂。