Bio-adhesives play a pivotal role in a wide range of medical applications. However, there are some problems about their application in different pH values and low adhesion force under wet conditions. Here, we report new recombinant fusion protein achieved by mussel foot proteins (Mfps) of Mytilus Californianus and gas vesicle protein A (GvpA) of Anabaena flos-aquae by genetic engineering methods. Transmission electron microscopy verified that these chimeric proteins self-assembled into β-sheet rich fibres because of GvpA amyloid structure. Also, in comparison to other studies, their adhesion forces were significantly increased, especially in the alkaline environments based on Mfp-3 and Mfp-5, confirmed by the colloidal probe atomic force microscope. This study illustrates that copolymer of Mfp-5-GvpA:GvpA-Mfp-3 can be used as a sturdy underwater adhesive with tolerance to auto-oxidation, especially at basic conditions.

生物粘合剂在广泛的医疗应用中发挥着关键作用。然而，它们在不同pH值下的应用和潮湿条件下的低附着力存在一些问题。在这里，我们报告了由加州贻贝的贻贝足蛋白 (Mfps)和鱼腥藻(Anabaena flos-aquae ) 的囊泡蛋白 A (GvpA)获得的新重组融合蛋白通过基因工程方法。透射电子显微镜证实，由于 GvpA 淀粉样蛋白结构，这些嵌合蛋白自组装成富含 β-折叠的纤维。此外，与其他研究相比，它们的粘附力显着增加，尤其是在基于 Mfp-3 和 Mfp-5 的碱性环境中，胶体探针原子力显微镜证实了这一点。该研究表明，Mfp-5-GvpA:GvpA-Mfp-3 的共聚物可用作坚固的水下粘合剂，具有自氧化耐受性，尤其是在碱性条件下。