Six new 11-mer peptaibols designed as zealpeptaibolins, A – F were isolated from the soil fungus, Trichoderma sp. RK10-F026, based on the principal component analysis of the MS data from five different culture compositions. Previously, 20-mer peptaibols from the same fungal strain were identified; 11-mer peptaibols in contrast were discovered from a different culture condition, signifying peptaibol production was culture condition-dependent. These peptaibols contained three Aib-Pro motifs in the sequence. The structures were established by NMR and HR-MS experiments including detailed MS/MS fragmentations. The absolute configurations were determined by Marfey’s analysis. Zealpeptaibolin F exhibited the strongest cytotoxicity toward K562 leukemia cells with an IC₅₀ value of 0.91 µM.

从土壤真菌木霉属植物中分离出六种新的 11 聚体 peptaibols，设计为 zealpeptaibolins，A - F。RK10-F026，基于来自五种不同培养成分的 MS 数据的主成分分析。以前，来自同一真菌菌株的 20-mer peptaibols 已被鉴定。相反，11-mer peptaibols 是从不同的培养条件中发现的，这表明 peptaibol 的生产依赖于培养条件。这些 peptaibols 在序列中包含三个 Aib-Pro 基序。结构是通过 NMR 和 HR-MS 实验建立的，包括详细的 MS/MS 碎片。绝对构型由 Marfey 分析确定。Zealpeptaibolin F 对 K562 白血病细胞表现出最强的细胞毒性，IC ₅₀值为 0.91 µM。