当前位置: X-MOL 学术J. Antibiot. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Zealpeptaibolin, an 11-mer cytotoxic peptaibol group with 3 Aib-Pro motifs isolated from Trichoderma sp. RK10-F026
The Journal of Antibiotics ( IF 2.1 ) Pub Date : 2021-06-23 , DOI: 10.1038/s41429-021-00429-y
Mira Syahfriena Amir Rawa 1, 2 , Toshihiko Nogawa 1 , Akiko Okano 1 , Yushi Futamura 1 , Habibah A Wahab 2 , Hiroyuki Osada 1
Affiliation  

Six new 11-mer peptaibols designed as zealpeptaibolins, A – F were isolated from the soil fungus, Trichoderma sp. RK10-F026, based on the principal component analysis of the MS data from five different culture compositions. Previously, 20-mer peptaibols from the same fungal strain were identified; 11-mer peptaibols in contrast were discovered from a different culture condition, signifying peptaibol production was culture condition-dependent. These peptaibols contained three Aib-Pro motifs in the sequence. The structures were established by NMR and HR-MS experiments including detailed MS/MS fragmentations. The absolute configurations were determined by Marfey’s analysis. Zealpeptaibolin F exhibited the strongest cytotoxicity toward K562 leukemia cells with an IC50 value of 0.91 µM.



中文翻译:

Zealpeptaibolin,一种 11 聚体细胞毒性 peptaibol 组,具有从木霉属 sp 中分离的 3 个 Aib-Pro 基序。RK10-F026

从土壤真菌木霉属植物中分离出六种新的 11 聚体 peptaibols,设计为 zealpeptaibolins,A - F。RK10-F026,基于来自五种不同培养成分的 MS 数据的主成分分析。以前,来自同一真菌菌株的 20-mer peptaibols 已被鉴定。相反,11-mer peptaibols 是从不同的培养条件中发现的,这表明 peptaibol 的生产依赖于培养条件。这些 peptaibols 在序列中包含三个 Aib-Pro 基序。结构是通过 NMR 和 HR-MS 实验建立的,包括详细的 MS/MS 碎片。绝对构型由 Marfey 分析确定。Zealpeptaibolin F 对 K562 白血病细胞表现出最强的细胞毒性,IC 50值为 0.91 µM。

更新日期:2021-06-23
down
wechat
bug