Residual biomass from surfactin production is a source of arginase and adsorbed surfactin that is useful for environmental remediation,World Journal of Microbiology and Biotechnology

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Residual biomass from surfactin production is a source of arginase and adsorbed surfactin that is useful for environmental remediation
World Journal of Microbiology and Biotechnology ( IF 4.0 ) Pub Date : 2021-06-23 , DOI: 10.1007/s11274-021-03094-3
Thais de Carvalho Silveira ₁ , Wyllerson Evaristo Gomes ₂ , Giovana Chinaglia Tonon ₃ , Thainá Godoy Beatto _{2,

3} , Nicolas Spogis ₃ , Luiz Henrique Dallan Cunha ₃ , Bruno Pera Lattaro ₃ , Alessandra Borin Nogueira ₃ , Renata Kelly Mendes _{2,

3} , Danillo Oliveira Alvarenga ₄ , Augusto Etchegaray _{1,

3}

Affiliation

Lipopeptides are important secondary metabolites produced by microbes. They find applications in environmental decontamination and in the chemical, pharmaceutical and food industries. However, their production is expensive. In the present work we propose three strategies to lower the production costs of surfactin. First, the coproduction of surfactin and arginase in a single growth. Second, extract the fraction of surfactin that adsorbs to the biomass and is removed from the growth medium through centrifugation. Third, use microbial biomass for the remediation of organic and inorganic contaminants. The coproduction of surfactin and arginase was evaluated by factorial design experiments using the LB medium supplemented with arginine. The best conditions for surfactin production were 22 h of growth at 37 °C using LB supplemented with arginine 7.3 g/L. Almost similar conditions were found to produce highest levels of arginase, 24 h and 6.45 g/L arginine. Decontamination of phenol and copper from artificial samples was attained by treatment with residues from lipopeptide production. Thus, cell suspensions and wash-waters used to extract surfactin from the biomass. Cell suspensions were used to successfully remove hydroquinone. Cell suspensions and wash-waters containing surfactin were successfully used to recover copper from solution. Specific monitoring methods were used for phenol and metal solutions, respectively a biosensor based on tyrosinase and either atomic absorption flame ionization spectrometry or absorbance coupled to the Arduino™ platform. Therefore, we report three alternative strategies to lower the production costs in lipopeptide production, which include the effective recovery of copper and phenol from contaminated waters using residues from surfactin production.

Graphic abstract

Sustainable and profitable production of surfactin can be achieved by a coproduction strategy of lipopeptides and enzymes. Lipopeptides are collected in the supernatant and enzymes in the biomass. In addition, lipopeptides that coprecipitate with biomass can be recovered by washing. Lipopeptide wash-waters find applications in remediation and cells can also be used for environmental decontamination.

中文翻译：

来自表面活性素生产的残留生物量是精氨酸酶和吸附表面活性素的来源，可用于环境修复

脂肽是微生物产生的重要次级代谢产物。它们可用于环境净化以及化学、制药和食品行业。然而，它们的生产是昂贵的。在目前的工作中，我们提出了三种降低表面活性素生产成本的策略。首先，在单一生长中同时产生表面活性素和精氨酸酶。其次，提取吸附到生物质上并通过离心从生长培养基中去除的表面活性素的部分。第三，利用微生物生物质修复有机和无机污染物。使用补充有精氨酸的 LB 培养基，通过析因设计实验评估表面活性素和精氨酸酶的共同产生。表面活性素生产的最佳条件是在 37°C 下使用补充有精氨酸 7 的 LB 生长 22 小时。3 克/升。发现几乎相似的条件产生最高水平的精氨酸酶，24 小时和 6.45 g/L 精氨酸。人工样品中苯酚和铜的净化是通过用脂肽生产中的残留物处理来实现的。因此，细胞悬浮液和洗涤水用于从生物质中提取表面活性素。细胞悬液用于成功去除对苯二酚。含有表面活性素的细胞悬浮液和洗涤水成功地用于从溶液中回收铜。特定的监测方法分别用于苯酚和金属溶液，分别是基于酪氨酸酶的生物传感器和原子吸收火焰电离光谱法或耦合到 Arduino™ 平台的吸光度。因此，我们报告了三种降低脂肽生产成本的替代策略，