The study aims to explore the interaction between miR-133a-3p and cell division cycle associated 8 (CDCA8) in oesophageal cancer (EC) and their effect on malignant behaviour of EC cells. Differential miRNAs and mRNAs were obtained from The Cancer Genome Atlas (TCGA) database. Quantitative real-time PCR (qRT-PCR) was used to detect the expression levels of miR-133a-3p and CDCA8 mRNA in EC cells. Western blot was used to detect the expression of CDCA8 protein. CCK-8, flow cytometry and Transwell assays were conducted to detect cell proliferation, cell cycle and apoptosis, as well as migration and invasion, respectively. The targeting relationship between miR-133a-3p and CDCA8 was verified by dual-luciferase reporter gene assay. In EC, miR-133a-3p expression was evidently low and CDCA8 expression was prominently high. MiR-133a-3p downregulated CDCA8 expression. A range of cell function experiments revealed that CDCA8 promoted the proliferation, migration and invasion of EC cells, reduced cell cycle arrest in G0/G1 phase and inhibited cell apoptosis, while miR-133a-3p could reverse the above effects by regulating CDCA8. MiR-133a-3p is a crucial tumour suppressor miRNA in EC, playing a tumour suppressor role by targeting CDCA8.

中文翻译：

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MiR-133a-3p 通过靶向 CDCA8 抑制食管癌的恶性进展

本研究旨在探讨食管癌（EC）中miR-133a-3p与细胞分裂周期相关8（CDCA8）的相互作用及其对EC细胞恶性行为的影响。差异 miRNA 和 mRNA 从癌症基因组图谱 (TCGA) 数据库中获得。定量实时PCR（qRT-PCR）用于检测EC细胞中miR-133a-3p和CDCA8 mRNA的表达水平。Western blot检测CDCA8蛋白的表达。CCK-8、流式细胞术和Transwell检测分别检测细胞增殖、细胞周期和凋亡，以及迁移和侵袭。miR-133a-3p和CDCA8之间的靶向关系通过双荧光素酶报告基因检测得到验证。在EC中，miR-133a-3p表达明显低，CDCA8表达明显高。MiR-133a-3p 下调 CDCA8 表达。一系列细胞功能实验表明，CDCA8促进EC细胞的增殖、迁移和侵袭，减少细胞周期停滞在G0/G1期，抑制细胞凋亡，而miR-133a-3p可以通过调节CDCA8逆转上述作用。MiR-133a-3p 是 EC 中重要的抑癌 miRNA，通过靶向 CDCA8 发挥抑癌作用。