1 PURPOSE AND APPROPRIATE SAMPLE TYPES

This 19-parameter, 18-color flow cytometry panel was designed and optimized to enable the comprehensive and simultaneous immunophenotyping of distinct T-cell, B-cell, and antibody secreting cell (ASC) subsets within murine tissues (Table 1). Cellular populations identified by using this OMIP include two major subsets of B-cells (memory and activated), two ASC subsets (plasma cells and plasmablasts), and seven major subsets of CD4⁺ T-cells (naïve, central memory, effector memory, helper, regulatory, follicular helper, and follicular regulatory). Staining was performed on freshly isolated splenocytes from 21-day-old BALB/c mice, however, due to the omission of mouse strain-specific markers, this OMIP can be implemented across a range of murine models where in-depth immunophenotyping of the diverse repertoire of T-cell, B-cell, and ASC populations is required.

TABLE 1. Summary table

Purpose	Comprehensive immunophenotyping of T-cell, B-cell, and ASC subsets
Species	Mouse
Cell types	Murine tissues containing lymphocyte populations
Cross-reference	OMIP-031, OMIP-032, OMIP-054, OMIP-061

中文翻译：

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OMIP 076：小鼠 T 细胞、B 细胞和抗体分泌细胞亚群的高维免疫表型分析

1 目的和适当的样本类型

这款 19 参数、18 色流式细胞仪面板经过设计和优化，可对小鼠组织内不同的 T 细胞、B 细胞和抗体分泌细胞 (ASC) 亚群进行全面和同时的免疫表型分析（表 1）。使用该 OMIP 鉴定的细胞群包括 B 细胞的两个主要亚群（记忆细胞和活化细胞）、两个 ASC 亚群（浆细胞和浆母细胞）和 CD4 ^{+的七个主要亚群}T 细胞（幼稚、中央记忆、效应记忆、辅助、调节、滤泡辅助和滤泡调节）。对来自 21 天大的 BALB/c 小鼠的新鲜分离的脾细胞进行染色，然而，由于省略了小鼠品系特异性标记，该 OMIP 可以在一系列小鼠模型中实施，其中对多种小鼠进行深入的免疫表型分析需要 T 细胞、B 细胞和 ASC 群体的所有组成部分。

表 1.汇总表

目的	T 细胞、B 细胞和 ASC 亚群的综合免疫表型分析
物种	鼠
细胞类型	含有淋巴细胞群的鼠组织
交叉引用	OMIP-031、OMIP-032、OMIP-054、OMIP-061