Background

Gliomas are common malignant tumors in the nervous system, known for poor prognosis and low survival rate.

Objective

This study aims to explore functions of miR-137 in glioma progression and identify messenger RNAs (mRNA) regulated by miR-137, which provides new ideas for further exploration of glioma therapeutic targets.

Methods

Gene expression data were downloaded from the Cancer Genome Atlas database, and abnormally expressed miRNAs and mRNAs in glioma were analyzed. The expression of genes in 20 pairs of clinical tissue samples and glioma cell lines were detected through qRT-PCR, and the expression of proteins was detected through Western blot. Changes in cell proliferative level after transfection were detected via CCK8 assay, and changes in cell migratory and invasive abilities were detected by Transwell assay. Besides, dual-luciferase reporter assay was employed to testify binding relationship between two genes.

Results

Our study found that miR-137 was significantly and lowly expressed in glioma tissue and cell lines, and the prognoses of glioma patients with highly expressed miR-137 were more optimistic. Overexpressed miR-137 could remarkably inhibit proliferative, invasive and migratory abilities of glioma cells U87, while transfection of miR-137 inhibitor presented an opposite effect. Additionally, EZH2 was a direct target of miR-137 and overexpressed EZH2 effectively reversed the effect of miR-137 on glioma proliferation and migration.

Conclusions

Our study found that miR-137 could suppress the proliferation, invasion and migration of glioma cells through regulating the expression of EZH2. So far, we have found a novel regulatory pair that influences glioma progression, providing a basis for further development of new therapeutic strategies.

中文翻译：

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MiR-137通过靶向调控EZH2抑制胶质瘤的增殖、侵袭和迁移

背景

胶质瘤是神经系统中常见的恶性肿瘤，以预后差、生存率低着称。

客观的

本研究旨在探索miR-137在胶质瘤进展中的作用，鉴定miR-137调控的信使RNA（mRNA），为进一步探索胶质瘤治疗靶点提供新思路。

方法

从Cancer Genome Atlas数据库下载基因表达数据，分析胶质瘤中异常表达的miRNA和mRNA。qRT-PCR检测20对临床组织样本和胶质瘤细胞系中基因的表达，Western blot检测蛋白的表达。CCK8法检测转染后细胞增殖水平的变化，Transwell法检测细胞迁移和侵袭能力的变化。此外，采用双荧光素酶报告基因测定来证明两个基因之间的结合关系。

结果

我们的研究发现miR-137在胶质瘤组织和细胞系中显着低表达，高表达miR-137的胶质瘤患者预后更乐观。过表达的miR-137可以显着抑制胶质瘤细胞U87的增殖、侵袭和迁移能力，而转染miR-137抑制剂则呈现相反的效果。此外，EZH2 是 miR-137 的直接靶标，过表达的 EZH2 有效地逆转了 miR-137 对胶质瘤增殖和迁移的影响。

结论

我们的研究发现，miR-137可以通过调节EZH2的表达来抑制胶质瘤细胞的增殖、侵袭和迁移。到目前为止，我们已经发现了一种影响神经胶质瘤进展的新型调节对，为进一步开发新的治疗策略提供了基础。