Quantitative phase measurements of human cell nuclei using X-ray ptychography,Journal of Synchrotron Radiation

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Quantitative phase measurements of human cell nuclei using X-ray ptychography
Journal of Synchrotron Radiation ( IF 2.4 ) Pub Date : 2021-06-23 , DOI: 10.1107/s1600577521004586
Jorg Schwenke ₁ , Mohammed Yusuf ₁ , Laura A Shemilt ₁ , Ulrich Wagner ₂ , Atiqa Sajid ₃ , Graeme R Morrison ₁ , Fucai Zhang ₁ , Aaron Parsons ₂ , Christoph Rau ₂ , Ian K Robinson ₁

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The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.

中文翻译：

使用 X 射线 ptychography 对人类细胞核进行定量相位测量

人类细胞核是保存生命遗传蓝图的重要细胞器。在这项工作中，X 射线 ptychography 用于使用其独特的相移信息评估人类细胞核的质量。测量是在钻石光源的 I13-1 光束线处进行的，该光源具有极大的横向相干特性。ptychographic 衍射成像方法允许对大型结构进行成像，从而对 2D 投影中的相移进行定量测量。在本文中，提出了一种改进的 ptychography 算法，该算法提高了弱散射样本的重建质量。还演示了这种方法在计算几个人类细胞核质量方面的应用。

更新日期：2021-07-24

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