In mammals, interleukin (IL)-17 receptor C (IL-17RC) and IL-17RA mediate IL-17A and IL-17F signaling to produce mucin, antimicrobial peptides, and maintain healthy intestinal flora. However, IL-17RC signaling in fish remains unclear. In this study, three il17rc transcripts (il17rca1, il17rca2, and il17rcb) from the Japanese medaka (Oryzias latipes) were cloned; il17rca1 and il17rca2 mRNAs were alternatively spliced from il17rca pre-mRNA as transcript variants. The il17rca and il17rcb genes were located on chromosomes 7 and 5, respectively. Teleost clades containing medaka il17rca and il17rcb clustered separately from the tetrapod clade. In adult tissues, il17rca1 expression was significantly higher than il17rca2 and il17rcb. Conversely, il17rcb expression was significantly higher in embryos and larvae. These expression patterns changed following infection with Edwardsiella piscicida and Aeromonas hydrophila. Furthermore, an immunoprecipitation assay using recombinant IL-17RCs and rIL-17A/Fs suggested that, in teleosts, three ligands could function in signaling through two IL-17RCs.

在哺乳动物中，白细胞介素 (IL)-17 受体 C (IL-17RC) 和 IL-17RA 介导 IL-17A 和 IL-17F 信号传导以产生粘蛋白、抗菌肽并维持健康的肠道菌群。然而，鱼类中的 IL-17RC 信号传导仍不清楚。在这项研究中，克隆了来自日本青鳉（Oryzias latipes ）的三个il17rc转录本（il17rca1、il17rca2和il17rcb ） ；il17rca1和il17rca2 mRNAs 从il17rca pre-mRNA 作为转录变体交替剪接。il17rca和il17rcb基因分别位于染色体 7 和 5。含有青鳉的硬骨鱼进化枝il17rca和il17rcb与四足动物进化枝分开聚集。在成人组织中，il17rca1 的表达明显高于il17rca2和il17rcb。相反，il17rcb在胚胎和幼虫中的表达明显更高。这些表达模式在感染爱德华氏菌和嗜水气单胞菌后发生了变化。此外，使用重组 IL-17RCs 和 rIL-17A/Fs 的免疫沉淀分析表明，在硬骨鱼中，三个配体可以通过两个 IL-17RCs 发挥信号传导作用。