Heat tolerance is important for the sustainable production of many crops, including chile pepper. Tolerance to high temperature is complex and involves various component traits, with pollen viability being among the most important. in vitro pollen assays for heat tolerance have been widely used in chile pepper; however, associations between the pollen treatment and pollination have not been widely explored. The objectives of this study were to validate the utility of in vitro heat stress pollen characterization through in vivo pollination during summer and winter seasons and to evaluate the cross-compatibility among wild and domesticated species to initiate introgression population development. Seven entries of wild and domestic Capsicum species grown during the summer and winter seasons were used to evaluate pollination success rate. Pollen was either used directly or treated at 38 °C for four hours before making reciprocal self- and cross-pollination among all the entries. Significant associations between in vitro pollen treatment and pollination success rate during summer and winter seasons were identified. Heat treatment was a greater contributor to variability than the growing environment, which validates previous reports on the usefulness of studying pollen in vitro in selection for heat tolerance. Accessions of the wild progenitor C. annuum var glabriusculum, PBC 1969 and PBC 1970, were identified as a potential heat-tolerant source for use in breeding and future research. This work provides a basis for future research in exploring additional heat tolerance components as well as for the development of phenotyping assays for pollen or other floral traits.

中文翻译：

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生长环境和热处理对智利辣椒（辣椒属）种内和种间授粉的影响

耐热性对于包括智利辣椒在内的许多作物的可持续生产很重要。对高温的耐受性是复杂的，涉及各种组成性状，其中最重要的是花粉活力。用于耐热性的体外花粉测定已广泛用于智利辣椒；然而，花粉处理和授粉之间的关联尚未得到广泛探索。本研究的目的是通过夏季和冬季的体内授粉来验证体外热应激花粉表征的效用，并评估野生和驯化物种之间的交叉相容性以启动基因渗入种群发展。野生和家养辣椒七项使用夏季和冬季生长的物种来评估授粉成功率。花粉要么直接使用，要么在 38°C 下处理四小时，然后在所有条目之间进行相互自花授粉和异花授粉。确定了夏季和冬季的体外花粉处理与授粉成功率之间的显着关联。与生长环境相比，热处理对变异性的影响更大，这证实了先前关于在体外研究花粉以选择耐热性的有用性的报告。野生祖先C 的种质。辣椒VAR glabriusculum、PBC 1969 和 PBC 1970 被确定为用于育种和未来研究的潜在耐热来源。这项工作为未来探索额外的耐热成分以及开发花粉或其他花卉性状的表型分析奠定了基础。