The use of deep eutectic solvents (DES) with buffer as cosolvent (up to 10 % v/v) leads to low-viscous media in which lipases can perform synthetic reactions, instead of hydrolysis. This paper explores the immobilization of Pseudomonas stutzeri lipase (TL) in cross-linking aggregates (CLEA) to deliver robust derivatives that are active in media like choline chloride – glycerol DES with buffer as cosolvent. While the free TL enzyme was markedly inactive in these media, TL-CLEA derivatives perform esterifications and can be reused several times. Overall, results are consistent with previous experiments reported for other lipases in these DES-water media and confirm that CLEA immobilization turns out a very useful and straightforward alternative for generating active (bio)catalysts for DES-aqueous media systems. Immobilized systems open the possibility of performing continuous processes in low-viscous DES-buffer media.

使用深共熔溶剂 (DES) 和缓冲液作为共溶剂（高达 10% v/v）会导致低粘度介质，脂肪酶可以在其中进行合成反应，而不是水解。本文探讨了施氏假单胞菌的固定化交联聚集体 (CLEA) 中的脂肪酶 (TL) 可提供稳定的衍生物，这些衍生物在氯化胆碱 - 甘油 DES 等介质中具有活性，缓冲液作为共溶剂。虽然游离 TL 酶在这些培养基中明显失活，但 TL-CLEA 衍生物会进行酯化反应并且可以多次重复使用。总体而言，结果与之前报道的在这些 DES-水介质中的其他脂肪酶的实验一致，并证实 CLEA 固定是一种非常有用和直接的替代方法，可用于生成 DES-水介质系统的活性（生物）催化剂。固定系统开启了在低粘度 DES 缓冲介质中进行连续过程的可能性。