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Immobilization of Pseudomonas stutzeri lipase through Cross-linking Aggregates (CLEA) for reactions in Deep Eutectic Solvents
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2021-06-23 , DOI: 10.1016/j.jbiotec.2021.06.021
Nadia Guajardo 1 , Katherine Ahumada 2 , Pablo Domínguez de María 3
Affiliation  

The use of deep eutectic solvents (DES) with buffer as cosolvent (up to 10 % v/v) leads to low-viscous media in which lipases can perform synthetic reactions, instead of hydrolysis. This paper explores the immobilization of Pseudomonas stutzeri lipase (TL) in cross-linking aggregates (CLEA) to deliver robust derivatives that are active in media like choline chloride – glycerol DES with buffer as cosolvent. While the free TL enzyme was markedly inactive in these media, TL-CLEA derivatives perform esterifications and can be reused several times. Overall, results are consistent with previous experiments reported for other lipases in these DES-water media and confirm that CLEA immobilization turns out a very useful and straightforward alternative for generating active (bio)catalysts for DES-aqueous media systems. Immobilized systems open the possibility of performing continuous processes in low-viscous DES-buffer media.



中文翻译:

通过交联聚集体 (CLEA) 固定施氏假单胞菌脂肪酶,用于在深共晶溶剂中进行反应

使用深共熔溶剂 (DES) 和缓冲液作为共溶剂(高达 10% v/v)会导致低粘度介质,脂肪酶可以在其中进行合成反应,而不是水解。本文探讨了施氏假单胞菌的固定化交联聚集体 (CLEA) 中的脂肪酶 (TL) 可提供稳定的衍生物,这些衍生物在氯化胆碱 - 甘油 DES 等介质中具有活性,缓冲液作为共溶剂。虽然游离 TL 酶在这些培养基中明显失活,但 TL-CLEA 衍生物会进行酯化反应并且可以多次重复使用。总体而言,结果与之前报道的在这些 DES-水介质中的其他脂肪酶的实验一致,并证实 CLEA 固定是一种非常有用和直接的替代方法,可用于生成 DES-水介质系统的活性(生物)催化剂。固定系统开启了在低粘度 DES 缓冲介质中进行连续过程的可能性。

更新日期:2021-06-28
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