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The hepatopancreas of the mangrove crab Neosarmatium africanum: a possible key to understanding the effects of wastewater exposure (Mayotte Island, Indian Ocean)
Environmental Science and Pollution Research Pub Date : 2021-06-23 , DOI: 10.1007/s11356-021-14892-5
Laura Mégevand 1 , Diana Martínez-Alarcón 2 , Dimitri Theuerkauff 3 , Georgina A Rivera-Ingraham 4 , Mathilde Lejeune 5 , Jehan-Hervé Lignot 1 , Elliott Sucré 1, 6
Affiliation  

Mangrove crabs are ecosystem engineers through their bioturbation activity. On Mayotte Island, the abundance of Neosarmatium africanum decreased in wastewater-impacted areas. Previous analyses showed that global crab metabolism is impacted by wastewater, with a burst in O2 consumption that may be caused by osmo-respiratory trade-offs since gill functioning was impacted. As the hepatopancreas is a key metabolic organ, the purpose of this study was to investigate the physiological effects of wastewater and ammonia-N 5-h exposure on crabs to better understand the potential trade-offs underlying the global metabolic state. Catalase, superoxide dismutase, glutathione S-transferase, total digestive protease, and serine protease (trypsin and chymotrypsin) activities were assessed. Histological analyses were performed to determine structural modifications. No effect of short-term wastewater and ammonia-N exposure was found in antioxidant defenses or digestive enzyme activity. However, histological changes of B-cells indicate an increase in intracellular digestive activity through higher vacuolization processes and tubule dilation in wastewater-exposed crabs.



中文翻译:

红树林蟹 Neosarmatium Africanum 的肝胰腺:了解废水暴露影响的可能关键(马约特岛,印度洋)

红树林蟹通过其生物扰动活动成为生态系统工程师。在马约特岛,受废水影响的地区,非洲新鱼藻的丰度下降。先前的分析表明,全球螃蟹代谢受到废水的影响,其中 O 2 激增由于鳃功能受到影响,因此可能由渗透呼吸权衡引起的消耗。由于肝胰腺是关键的代谢器官,本研究的目的是研究废水和氨氮 5 小时暴露对螃蟹的生理影响,以更好地了解全球代谢状态的潜在权衡。评估了过氧化氢酶、超氧化物歧化酶、谷胱甘肽 S-转移酶、总消化蛋白酶和丝氨酸蛋白酶(胰蛋白酶和糜蛋白酶)的活性。进行组织学分析以确定结构修饰。短期废水和氨氮暴露对抗氧化防御或消化酶活性没有影响。然而,

更新日期:2021-06-23
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