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Infrared Plasmonic Biosensor with Tetrahedral DNA Nanostructure as Carriers for Label-Free and Ultrasensitive Detection of miR-155
Advanced Science ( IF 14.3 ) Pub Date : 2021-06-21 , DOI: 10.1002/advs.202100583 Xindan Hui 1 , Cheng Yang 2 , Dongxiao Li 1 , Xianming He 1 , He Huang 1 , Hong Zhou 1, 3 , Ming Chen 2 , Chengkuo Lee 3 , Xiaojing Mu 1
Advanced Science ( IF 14.3 ) Pub Date : 2021-06-21 , DOI: 10.1002/advs.202100583 Xindan Hui 1 , Cheng Yang 2 , Dongxiao Li 1 , Xianming He 1 , He Huang 1 , Hong Zhou 1, 3 , Ming Chen 2 , Chengkuo Lee 3 , Xiaojing Mu 1
Affiliation
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MicroRNAs play an important role in early development, cell proliferation, apoptosis, and cell death, and are aberrantly expressed in many types of cancers. To understand their function and diagnose cancer at an early stage, it is crucial to quantitatively detect microRNA without invasive labels. Here, a plasmonic biosensor based on surface-enhanced infrared absorption (SEIRA) for rapid, label-free, and ultrasensitive detection of miR-155 is reported. This technology leverages metamaterial perfect absorbers stimulating the SEIRA effect to provide up to 1000-fold near-field intensity enhancement over the microRNA fingerprint spectral bands. Additionally, it is discovered that the limit of detection (LOD) of the biosensor can be greatly improved by using tetrahedral DNA nanostructure (TDN) as carriers. By using near-field enhancement of SEIRA and specific binding of TDN, the biosensor achieves label-free detection of miR-155 with a high sensitivity of 1.162% pm−1 and an excellent LOD of 100 × 10−15 m. The LOD is about 5000 times lower than that using DNA single strand as probes and about 100 times lower than that of the fluorescence detection method. This work can not only provide a powerful diagnosis tool for the microRNAs detection but also gain new insights into the field of label-free and ultrasensitive SEIRA-based biosensing.
中文翻译：
以四面体 DNA 纳米结构为载体的红外等离子生物传感器用于 miR-155 的无标记和超灵敏检测
微小RNA在早期发育、细胞增殖、凋亡和细胞死亡中起重要作用，并且在许多类型的癌症中异常表达。为了了解它们的功能并在早期诊断癌症，在没有侵入性标记的情况下定量检测 microRNA 至关重要。在这里，一种基于表面增强红外吸收 (SEIRA) 的等离子体生物传感器用于快速、无标记和超灵敏检测miR-155被报道。该技术利用超材料完美吸收剂刺激 SEIRA 效应，在 microRNA 指纹光谱带上提供高达 1000 倍的近场强度增强。此外，发现使用四面体DNA纳米结构（TDN）作为载体可以大大提高生物传感器的检测限（LOD）。通过使用SEIRA的近场增强和TDN的特异性结合，该生物传感器实现了miR-155的无标记检测，具有1.162% p m -1的高灵敏度和100 × 10 -15 m的优异LOD. LOD比使用DNA单链作为探针低约5000倍，比荧光检测方法低约100倍。这项工作不仅可以为 microRNAs 检测提供强大的诊断工具，还可以为无标记和超灵敏的基于 SEIRA 的生物传感领域提供新的见解。
更新日期：2021-08-19
中文翻译：
以四面体 DNA 纳米结构为载体的红外等离子生物传感器用于 miR-155 的无标记和超灵敏检测
微小RNA在早期发育、细胞增殖、凋亡和细胞死亡中起重要作用，并且在许多类型的癌症中异常表达。为了了解它们的功能并在早期诊断癌症，在没有侵入性标记的情况下定量检测 microRNA 至关重要。在这里，一种基于表面增强红外吸收 (SEIRA) 的等离子体生物传感器用于快速、无标记和超灵敏检测miR-155被报道。该技术利用超材料完美吸收剂刺激 SEIRA 效应，在 microRNA 指纹光谱带上提供高达 1000 倍的近场强度增强。此外，发现使用四面体DNA纳米结构（TDN）作为载体可以大大提高生物传感器的检测限（LOD）。通过使用SEIRA的近场增强和TDN的特异性结合，该生物传感器实现了miR-155的无标记检测，具有1.162% p m -1的高灵敏度和100 × 10 -15 m的优异LOD. LOD比使用DNA单链作为探针低约5000倍，比荧光检测方法低约100倍。这项工作不仅可以为 microRNAs 检测提供强大的诊断工具，还可以为无标记和超灵敏的基于 SEIRA 的生物传感领域提供新的见解。
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