ABSTRACT

In this paper, bovine serum albumin served as the initial materials for preparing a kind of cyan-emitting fluorescent protein nanoparticles (BSA NPs). The BSA NPs were further adopted as a template to synthesize protein nanoparticles-stabilized silver nanoclusters (BSA NPs@Ag NCs) that offered two well-separated fluorescence emission peaks respectively at 470 nm and 670 nm. Dopamine could specifically induce the fluorescence enhancement at 470 nm and the fluorescence quenching at 670 nm of BSA NPs@Ag NCs. Moreover, cupric ions could cause the fluorescence quenching at 670 nm of BSA NPs@Ag NCs just with a negligible impact on the fluorescence intensity at 470 nm. The BSA NPs@Ag NCs can serve as a ratiometric probe for detection of dopamine and cupric ions.

摘要

本文以牛血清白蛋白为初始材料制备了一种发青色荧光蛋白纳米粒子（BSA NPs）。进一步采用 BSA NPs 作为模板合成蛋白质纳米粒子稳定的银纳米团簇 (BSA NPs@Ag NCs)，其分别在 470 nm 和 670 nm 处提供两个分离良好的荧光发射峰。多巴胺可以特异性地诱导 BSA NPs@Ag NCs 在 470 nm 处的荧光增强和在 670 nm 处的荧光猝灭。此外，铜离子可能导致 BSA NPs@Ag NCs 在 670 nm 处的荧光猝灭，而对 470 nm 处的荧光强度的影响可以忽略不计。BSA NPs@Ag NCs 可用作检测多巴胺和铜离子的比率探针。