We explored multiple experimental treatments for regenerating tissue-cultured shoots of tetraploid Robinia pseudoacacia L., and assessed the most suitable combination of treatments for the development of a highly efficient regeneration system. Leaflets of tissue-cultured shoots were used as explants to study the effects of four different treatments on leaf regeneration capacity. Treatments included leaflet position on donor leaves, light conditions during culturing, the orientation of leaves on the growth medium, and the number of cut wounds per leaf. After determining an appropriate stem explant culture period, we assessed the influence of four treatments on regenerative capacity. The 35-d-old leaf explants produced the largest amount of callus tissue, while the highest average callus growth index was observed on the leaves cultured for an additional 30 d. Each leaf produced an average of five adventitious buds. The greatest numbers of buds and callus tissues were observed in the leaves collected from the uppermost position on the shoot, treated with six cut wounds per leaf, and grown under illuminated conditions.

我们探索了多种用于再生四倍体刺槐组织培养枝条的实验处理方法L.，并评估了开发高效再生系统的最合适的处理组合。使用组织培养芽的小叶作为外植体来研究四种不同处理对叶片再生能力的影响。处理包括供体叶子上的小叶位置、培养期间的光照条件、生长培养基上的叶子方向以及每片叶子上的切割伤口数。在确定合适的茎外植体培养期后，我们评估了四种处理对再生能力的影响。35 d 龄叶外植体产生最多的愈伤组织，而在再培养 30 d 的叶片上观察到最高的平均愈伤组织生长指数。每片叶子平均产生五个不定芽。