SummaryTo enhance the developmental competency of murine ovarian follicles cultured in vitro, C-type natriuretic peptide (CNP) was supplemented in the culture system. Although the mechanism is not fully elucidated, it was reported that the effect of CNP supplementation was mediated by increased cyclic guanosine monophosphate (cGMP). In the present study, cGMP levels in media for murine preantral follicle culture were compared both between a control group without CNP supplementation and an experimental group with CNP supplementation and between days in each group. In addition, follicle growth patterns and oocyte maturity were assessed and compared between the two groups. Results demonstrated that along with in vitro culture, cGMP levels increased (P < 0.05) both in the control group and the experimental group, whereas cGMP levels were not significantly different between the two groups on the same day of in vitro culture (P > 0.05). The oocyte’s maturity was superior in the experimental group compared with the control group (P < 0.05). As ovarian follicles grew three-dimensionally in the experimental group but were flattened in the control group, CNP might improve oocyte maturity through maintaining the three-dimensional architecture of the ovarian follicle because of increased transzonal projections (TZP) and functional gap junctions between oocyte and surrounding granulosa cells.

中文翻译：

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补充和不补充C型利钠肽的小鼠窦前卵泡体外生长期培养基中cGMP水平的评价

摘要提高培养的小鼠卵巢卵泡的发育能力体外，在培养系统中补充了C型利钠肽（CNP）。尽管该机制尚未完全阐明，但据报道，补充 CNP 的作用是由增加的环磷酸鸟苷 (cGMP) 介导的。在本研究中，比较了未添加 CNP 的对照组和添加 CNP 的实验组以及各组天数之间的小鼠窦前卵泡培养培养基中的 cGMP 水平。此外，评估和比较两组之间的卵泡生长模式和卵母细胞成熟度。结果表明，随着体外培养，cGMP 水平增加（磷< 0.05) 对照组和实验组, 而 cGMP 水平在同一天两组之间没有显着差异体外文化 （磷> 0.05)。实验组卵母细胞成熟度优于对照组（磷< 0.05)。由于实验组的卵泡呈三维生长，而对照组的卵泡变平，CNP 可能通过维持卵巢卵泡的三维结构来提高卵母细胞成熟度，因为它增加了跨区域投射 (TZP) 和卵母细胞与卵母细胞之间的功能间隙连接。周围的颗粒细胞。