SummaryThis study examined the effect of a cryoprotectant with and without pentoxifylline supplementation on the motility and viability of human testicular sperm, both before and after freezing. Testicular samples were obtained from 68 patients with azoospermia who came to the Andrology Service of West China Second University Hospital, Sichuan University, for testicular biopsies from December 2019 to April 2020. All patients were assigned randomly to two groups: experimental, whose testicular sperm were added to the cryoprotectant with pentoxifylline, and the control, whose testicular sperm were added to the cryoprotectant without pentoxifylline. Both groups used the same freezing and thawing methods. Testicular sperm motility in the experimental group was significantly higher than that of the control group, both before and after cryopreservation. The recovery rate of sperm motility in the experimental group was significantly higher than that of the control group. The percentage of samples with motile testicular sperm in the experimental group was significantly higher than that of the control group after thawing. Sperm viability was unchanged between the experimental and control groups, both before and after freezing. Overall, a pentoxifylline-supplemented cryoprotectant can significantly improve the motility of testicular sperm before and after cryopreservation.

中文翻译：

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一种添加己酮可可碱的冷冻保护剂可改善冷冻对人睾丸精子活力的影响

总结本研究检查了冷冻保护剂在冷冻前后添加和不添加己酮可可碱对人类睾丸精子活力和活力的影响。睾丸样本取自 2019 年 12 月至 2020 年 4 月在四川大学华西第二医院男科就诊的 68 例无精子症患者进行睾丸活检。所有患者随机分为两组：实验组，其睾丸精子添加到含有己酮可可碱的冷冻保护剂中，以及将睾丸精子添加到不含己酮可可碱的冷冻保护剂中的对照。两组均使用相同的冷冻和解冻方法。冷冻保存前后，实验组睾丸精子活力明显高于对照组。实验组精子活力恢复率明显高于对照组。解冻后，实验组具有活动睾丸精子的样本百分比显着高于对照组。冷冻前后精子活力在实验组和对照组之间没有变化。总体而言，补充己酮可可碱的冷冻保护剂可以显着提高冷冻保存前后睾丸精子的活力。冷冻前后精子活力在实验组和对照组之间没有变化。总体而言，补充己酮可可碱的冷冻保护剂可以显着提高冷冻保存前后睾丸精子的活力。冷冻前后精子活力在实验组和对照组之间没有变化。总体而言，补充己酮可可碱的冷冻保护剂可以显着提高冷冻保存前后睾丸精子的活力。