Vitreous humor (VH) is an alternative biological matrix with a great advantage of longer availability for analysis due to the lack of many enzymes. The use of VH in forensic toxicology may have an added benefit, however, this application requires rapid, simple, non-destructive, and relatively portable analytical analysis methods. These requirements may be met by Fourier transform infrared spectroscopy technique (FT-IR) equipped with attenuated total reflection accessory (ATR). FT-IR spectra of vitreous humor samples, deposited on glass slides, were collected and subsequent chemometric data analysis by means of Hierarchical Cluster Analysis and Principal Component Analysis was conducted. Differences between animal and human VH samples and human VH samples stored for diverse periods of time were detected. A kinetic study of changes in the VH composition up to 2 weeks showed the distinction of FT-IR spectra collected on the 1st and 14th day of storage. In addition, data obtained for the most recent human vitreous humor samples—collected 3 and 2 years before the study, presented successful discrimination of all time points studied. The method introduced was unable to detect mephedrone addition to VH in the concentration of 10 µg/cm³.

Graphic abstract

中文翻译：

---

光谱学作为一种有用的工具，用于鉴定法医毒理学目的的验尸玻璃体液随时间的变化

玻璃体液 (VH) 是一种替代生物基质，由于缺乏许多酶，因此具有更长的分析可用性的巨大优势。在法医毒理学中使用 VH 可能有额外的好处，但是，该应用需要快速、简单、无损且相对便携的分析分析方法。配备衰减全反射附件 (ATR) 的傅里叶变换红外光谱技术 (FT-IR) 可以满足这些要求。收集沉积在载玻片上的玻璃体液样品的 FT-IR 光谱，并通过分层聚类分析和主成分分析进行随后的化学计量数据分析。检测了动物和人类 VH 样品与储存不同时间段的人类 VH 样品之间的差异。长达 2 周的 VH 组成变化的动力学研究表明，在储存的第 1 天和第 14 天收集的 FT-IR 光谱存在差异。此外，在研究前 3 年和 2 年收集的最新人类玻璃体液样本的数据显示了对所有研究时间点的成功区分。引入的方法无法检测到 VH 中添加浓度为 10 µg/cm 的甲氧麻黄酮³ .

图形摘要