ABSTRACT

In this study, we designed and synthesized an implantable anti-CD25 antibody-immobilized polyethylene (CD25-PE) mesh to suppress tumor growth by removing regulatory T cells (Tregs). The PE mesh was graft-polymerized with poly(acrylic acid), and the anti-mouse CD25 antibody was then immobilized using the 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide reaction. Immobilization of the antibody on the PE mesh was confirmed by immunostaining. The CD25-PE mesh could effectively and selectively capture CD25-positive cells through antigen-antibody interactions when the CD25-PE mesh was incubated with a suspension of mouse spleen cells, including CD25-positive cells. In addition, implantation of the CD25-PE mesh into mice subcutaneously demonstrated the Treg-capturing ability of the CD25-PE mesh with only a weak inflammatory reaction. In tumor-bearing mice, tumor growth was suppressed by subcutaneous implantation of the CD25-PE mesh near the tumor for 1 week. These results suggested that the anti-CD25 antibody-immobilized material could capture Tregs in vivo and inhibit tumor proliferation in a limited tumor-bearing mouse model. Further research is needed to facilitate cancer immunotherapy using implantable anti-CD25 antibody-immobilized material as a Treg-capturing device.

摘要

在这项研究中，我们设计并合成了一种可植入的抗 CD25 抗体固定化聚乙烯 (CD25-PE) 网状物，通过去除调节性 T 细胞 (Tregs) 来抑制肿瘤生长。PE网与聚（丙烯酸）接枝聚合，然后使用1-乙基-3-（3-二甲基氨基丙基）-碳二亚胺反应固定抗小鼠CD25抗体。通过免疫染色确认抗体在 PE 网上的固定化。当 CD25-PE 网与小鼠脾细胞（包括 CD25 阳性细胞）悬液一起孵育时，CD25-PE 网可以通过抗原-抗体相互作用有效且选择性地捕获 CD25 阳性细胞。此外，将 CD25-PE 网植入小鼠皮下证明了 CD25-PE 网的 Treg 捕获能力，只有微弱的炎症反应。在荷瘤小鼠中，通过在肿瘤附近皮下植入 CD25-PE 网 1 周来抑制肿瘤生长。这些结果表明，抗 CD25 抗体固定化材料可以在体内捕获 Tregs 并在有限的荷瘤小鼠模型中抑制肿瘤增殖。需要进一步研究以使用可植入的抗 CD25 抗体固定材料作为 Treg 捕获装置促进癌症免疫治疗。