ABSTRACT

Sixteen polymorphic nuclear microsatellite markers were developed for Meliosma arnottiana (Wright) Walp. (Sabiaceae), a deciduous or half-evergreen tree. In Japan, the species is disjunctively distributed in the Izu Islands and the southern part of the Japanese archipelago, and the Izu population (var. hachijoensis) is regarded as a threatened species. Variations in the 16 markers were compared between the two populations on the Izu and Ryukyu Islands. The mean number of alleles was 3.00 and 5.31, and the mean expected heterozygosity was 0.233 and 0.513, respectively, on the Izu and Ryukyu Islands, and the genetic differentiation between the two populations was statistically significant. Of the 16 primer sets, five were amplified in all three congeneric species M. myriantha, M. rigida and M. tenuis, and eight or nine were amplified in each species. These newly developed microsatellite markers will be used to evaluate genetic diversity and genetic differentiation in M. arnottiana populations and are potentially useful for population genetic studies on congeneric species.

摘要

为Meliosma arnottiana (Wright) Walp开发了 16 个多态核微卫星标记。(Sabiaceae)，落叶或半常绿乔木。在日本，该物种间断分布于伊豆群岛和日本列岛南部，伊豆种群 (var. hachijoensis ) 被视为受威胁物种。比较了伊豆和琉球群岛两个种群之间 16 个标记的变化。伊豆和琉球群岛的等位基因平均数为3.00和5.31，平均预期杂合度分别为0.233和0.513，两个种群之间的遗传分化具有统计学意义。在 16 组引物中，有 5 组在所有三个同类物种中均被扩增M. myriantha、M.rigida和M. tenuis，每个物种中扩增了八九个。这些新开发的微卫星标记将用于评估M. arnottiana种群的遗传多样性和遗传分化，并可能对同类物种的种群遗传研究有用。