Abstract

The main reason for the failure of glaucoma drainage devices is the chronic inflammatory reaction caused by the poor biocompatibility of biomaterials, which leads to the migration and proliferation of scleral fibroblasts surrounding the devices thus forming the encapsulation that will block the aqueous humor drainage channel. In order to inhibit the reaction of scleral fibroblasts after glaucoma drainage device implantation, this study designed and fabricated a 5-fluorouracil (5-FU) loaded chitosan microtube (CMT) for glaucoma aqueous humor drainage. The CMTs were made by template based adsorption-precipitation method using chitosan with excellent biocompatibility as raw material due to its characteristic of pH dependent solubility. The physical properties of CMTs were investigated. The in vitro and in vivo biocompatibilities were studied as well. The developed drainage device is expected to possess the dual function of reducing intraocular pressure and inhibiting excessive fibrosis after glaucoma drainage device implantation, thereby providing a novel way for the research of glaucoma drainage devices.

摘要

青光眼引流装置失败的主要原因是生物材料的生物相容性差引起慢性炎症反应，导致装置周围巩膜成纤维细胞迁移增殖，形成包膜，堵塞房水引流通道。为了抑制青光眼引流装置植入后巩膜成纤维细胞的反应，本研究设计并制作了一种载有5-氟尿嘧啶（5-FU）的壳聚糖微管（CMT）用于青光眼房水引流。CMTs 采用基于模板的吸附-沉淀法制备，以具有优异生物相容性的壳聚糖为原料，由于其具有 pH 依赖性溶解度的特性。研究了 CMT 的物理性质。在体外和还研究了体内生物相容性。所开发的引流器有望在青光眼引流器植入后具有降低眼压和抑制过度纤维化的双重功能，为青光眼引流器的研究提供新途径。