Abstract

Surface properties of octavinyl polyhedral oligomeric silsesquioxane (OVS), grafted polyurethane (PU) and heparinized carbon nanotubes (HCNTs) nanocomposite thin-films were evaluated, and the effect of OVS presence on platelet adhesion on the surface of nanocomposite films for artificial heart valve application was investigated using in-vitro tests. OVS was synthesized, then PU and CNT (PU/CNT) nanocomposite films were dipped in the solution of OVS in toluene for grafting. Synthesized OVS was investigated by XRD, FTIR, and NMR tests. SEM-EDX micrographs showed that the morphology of the PU/HCNT surface was obviously changed due to OVS grafting. Surface studies proved OVS grafting on the surface. Surface roughness was increased after grafting PU/HCNT surface with OVS in comparison to the untreated PU/HCNT nanocomposite surface. The contact angle was increased from 88° for PU/HCNT sample to 165° for OVS-grafted PU/HCNT sample. After the addition of CNTs to the matrix, the modulus was increased. According to the MTT test, modified polymeric nanocomposite has no cytotoxicity, and the adhesion of L929 fibroblast cells on the grafted surface was increased. Its shown in the platelet adhesion test that blood compatibility of the OVS grafted films are increased in comparison with PU/HCNT. Surface calcification of OVS-grafted PU/HCNT was reduced according to the in-vitro calcification test. It has been concluded that the combination of physical and mechanical properties of PU/HCNT and the calcification resistance of OVS is a suitable achievement for heart valve application.

摘要

评价了八乙烯基多面体低聚倍半硅氧烷 (OVS)、接枝聚氨酯 (PU) 和肝素化碳纳米管 (HCNT) 纳米复合薄膜的表面性质，以及 OVS 的存在对用于人工心脏瓣膜的纳米复合薄膜表面血小板粘附的影响使用体外测试进行了研究。合成OVS，然后将PU和CNT（PU/CNT）纳米复合薄膜浸入OVS的甲苯溶液中进行接枝。通过 XRD、FTIR 和 NMR 测试对合成的 OVS 进行了研究。SEM-EDX显微照片显示，由于OVS接枝，PU/HCNT表面的形貌发生了明显变化。表面研究证明OVS接枝在表面上。与未处理的 PU/HCNT 纳米复合材料表面相比，用 OVS 接枝 PU/HCNT 表面后表面粗糙度增加。接触角从 PU/HCNT 样品的 88° 增加到 OVS 接枝的 PU/HCNT 样品的 165°。在向基体中添加 CNT 后，模量增加。根据MTT测试，改性聚合物纳米复合材料没有细胞毒性，L929成纤维细胞在移植表面的粘附性增加。血小板粘附试验表明，与PU/HCNT相比，OVS接枝膜的血液相容性有所提高。根据体外钙化试验，OVS 接枝 PU/HCNT 的表面钙化减少。已经得出结论，PU/HCNT的物理和机械性能与OVS的钙化抗性相结合是心脏瓣膜应用的合适成就。在向基体中添加 CNT 后，模量增加。根据MTT测试，改性聚合物纳米复合材料没有细胞毒性，L929成纤维细胞在移植表面的粘附性增加。血小板粘附试验表明，与PU/HCNT相比，OVS接枝膜的血液相容性有所提高。根据体外钙化试验，OVS 接枝 PU/HCNT 的表面钙化减少。已经得出结论，PU/HCNT的物理和机械性能与OVS的钙化抗性相结合是心脏瓣膜应用的合适成就。在向基体中添加 CNT 后，模量增加。根据MTT测试，改性聚合物纳米复合材料没有细胞毒性，L929成纤维细胞在移植表面的粘附性增加。血小板粘附试验表明，与PU/HCNT相比，OVS接枝膜的血液相容性有所提高。根据体外钙化试验，OVS 接枝 PU/HCNT 的表面钙化减少。已经得出结论，PU/HCNT的物理和机械性能与OVS的钙化抗性相结合是心脏瓣膜应用的合适成就。血小板粘附试验表明，与PU/HCNT相比，OVS接枝膜的血液相容性有所提高。根据体外钙化试验，OVS 接枝 PU/HCNT 的表面钙化减少。已经得出结论，PU/HCNT的物理和机械性能与OVS的钙化抗性相结合是心脏瓣膜应用的合适成就。血小板粘附试验表明，与PU/HCNT相比，OVS接枝膜的血液相容性有所提高。根据体外钙化试验，OVS 接枝 PU/HCNT 的表面钙化减少。已经得出结论，PU/HCNT的物理和机械性能与OVS的钙化抗性相结合是心脏瓣膜应用的合适成就。