Glands of Drosera absorb and transport nutrients from captured prey, but the mechanism and dynamics remain unclear. In this study, we offered animal proteins in the form of fluorescent albumin (FITC-BSA) and observed the reactions of the glands by live cell imaging and fluorescence microscopy. The ultrastructure of these highly dynamic processes was also assessed in high-pressure frozen and freeze substituted (HPF-FS) cells. HPF-FS yielded excellent preservation of the cytoplasm of all cell types, although the cytosol looked different in gland cells as compared to endodermoid and stalk cells. Especially prominent were the ER and its contacts with the plasma membrane, plasmodesmata, and other organelles as well as continuities between organelles. Also distinct were actin microfilaments in association with ER and organelles. Application of FITC-BSA to glands caused the formation of fluorescent endosomes that pinched off the plasma membrane. Endosomes fused to larger aggregates, and accumulated in the bulk cytoplasm around the nucleus. They did not fuse with the cell sap vacuole but remained for at least three days; in addition, fluorescent vesicles also proceeded through endodermoid and transfer cells to the epidermal and parenchymal cells of the tentacle stalk.

茅膏菜腺从捕获的猎物中吸收和运输营养物质，但机制和动力学仍不清楚。在这项研究中，我们提供了荧光白蛋白 (FITC-BSA) 形式的动物蛋白，并通过活细胞成像和荧光显微镜观察了腺体的反应。这些高度动态过程的超微结构也在高压冷冻和冷冻替代 (HPF-FS) 细胞中进行了评估。HPF-FS 对所有细胞类型的细胞质进行了极好的保存，尽管与内皮样细胞和茎细胞相比，腺细胞中的细胞质看起来不同。特别突出的是 ER 及其与质膜、胞间连丝和其他细胞器的接触以及细胞器之间的连续性。与 ER 和细胞器相关的肌动蛋白微丝也不同。将 FITC-BSA 应用于腺体导致形成夹断质膜的荧光内体。内体融合成更大的聚集体，并在细胞核周围的大量细胞质中积累。它们没有与细胞液泡融合，但至少保留了三天；此外，荧光囊泡还通过内皮样细胞转移到触手柄的表皮和实质细胞。