Phytopathogenic fungi in the order Diaporthales (Sordariomycetes) cause diseases on numerous economically important crops worldwide. In this study, we reassessed the diaporthalean species associated with prominent diseases of strawberry, namely leaf blight, leaf blotch, root rot and petiole blight, based on molecular data and morphological characters using fresh and herbarium collections. Combined analyses of four nuclear loci, 28S ribosomal DNA/large subunit rDNA (LSU), ribosomal internal transcribed spacers 1 and 2 with 5.8S ribosomal DNA (ITS), partial sequences of second largest subunit of RNA polymerase II (RPB2) and translation elongation factor 1-α (TEF1), were used to reconstruct a phylogeny for these pathogens. Results confirmed that the leaf blight pathogen formerly known as Phomopsis obscurans belongs in the family Melanconiellaceae and not with Diaporthe (syn. Phomopsis) or any other known genus in the order. A new genus Paraphomopsis is introduced herein with a new combination, Paraphomopsis obscurans, to accommodate the leaf blight fungus. Gnomoniopsis fragariae comb. nov. (Gnomoniaceae), is introduced to accommodate Gnomoniopsis fructicola, the cause of leaf blotch of strawberry. Both of the fungi causing leaf blight and leaf blotch were epitypified. Fresh collections and new molecular data were incorporated for Paragnomonia fragariae (Sydowiellaceae), which causes petiole blight and root rot of strawberry and is distinct from the above taxa. An updated multilocus phylogeny for the Diaporthales is provided with representatives of currently known families.

中文翻译：

---

与草莓相关的 diaporthalean 真菌的分子重新评估，包括叶枯病真菌，Paraphomopsis obscurans gen。等梳子。十一月 (黑木兰科)

Diaporthales (Sordariomycetes) 目中的植物病原真菌在全世界许多具有重要经济意义的作物上引起疾病。在这项研究中，我们使用新鲜和植物标本收集的分子数据和形态特征，重新评估了与草莓主要病害相关的 diaporthalean 物种，即叶枯病、叶斑病、根腐病和叶柄枯萎病。四个核位点、28S 核糖体 DNA/大亚基 rDNA (LSU)、具有 5.8S 核糖体 DNA (ITS) 的核糖体内部转录间隔区 1 和 2、RNA 聚合酶 II (RPB2) 的第二大亚基的部分序列和翻译延伸的组合分析因子 1-α (TEF1) 用于重建这些病原体的系统发育。结果证实，以前称为 Phomopsis obscurans 的叶枯病病原体属于 Melanconiellaceae 科，而不属于 Diaporthe (syn. Phomopsis) 或该目中的任何其他已知属。本文引入了一个新属 Paraphomopsis 和一个新的组合，Paraphomopsis obscurans，以适应叶枯病真菌。Gnomoniopsis fragariae 梳子。十一月 (Gnomoniaceae), 被引入以适应草莓叶斑病的原因 Gnomoniopsis fructicola。引起叶枯病和叶斑病的真菌都是表型化的。新的收藏品和新的分子数据被整合到 Paragnomonia fragariae (Sydowiellaceae)，它导致草莓的叶柄枯萎病和根腐病，与上述分类群不同。