This study investigated whether mixing low viscosity alginic acid with calcium phosphate cement (CPC) causes interconnected porosity in the CPC and enhances bone replacement by improving the biological interactions. Furthermore, we hypothesized that low viscosity alginic acid would shorten the setting time of CPC and improve its strength. CPC samples were prepared with 0, 5, 10, and 20% low viscosity alginic acid. After immersion in acetate buffer, possible porosification in CPC was monitored in vitro using scanning electron microscopy (SEM), and the setting times and compressive strengths were measured. In vivo study was conducted by placing CPC in a hole created on the femur of New Zealand white rabbit. Microcomputed tomography and histological examination were performed 6 weeks after implantation. SEM images confirmed that alginic acid enhanced the porosity of CPC compared to the control, and the setting time and compressive strength also improved. When incorporating a maximum amount of alginic acid, the new bone mass was significantly higher than the control group (P = 0.0153). These biological responses are promising for the translation of these biomaterials and their commercialization for clinic applications.

本研究调查了将低粘度海藻酸与磷酸钙骨水泥 (CPC) 混合是否会导致 CPC 中相互关联的孔隙率，并通过改善生物相互作用来增强骨替代。此外，我们假设低粘度海藻酸会缩短 CPC 的凝固时间并提高其强度。CPC 样品是用 0、5、10 和 20% 的低粘度海藻酸制备的。浸入醋酸盐缓冲液后，使用扫描电子显微镜 (SEM) 在体外监测 CPC 中可能的孔隙化，并测量凝固时间和抗压强度。体内研究是通过将 CPC 置于新西兰白兔股骨上形成的孔中进行的。植入后 6 周进行显微计算机断层扫描和组织学检查。SEM 图像证实，与对照相比，海藻酸提高了 CPC 的孔隙率，凝固时间和抗压强度也有所提高。当加入最大量的海藻酸时，新骨量明显高于对照组（P  = 0.0153）。这些生物反应有望用于这些生物材料的转化及其临床应用的商业化。