Methicillin-resistant and Vancomycin-resistant Staphylococcus aureus bacteria (MRSA and VRSA, respectively) can seriously jeopardizes bone implants. This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections. Chitosan/bioactive glass nanoparticles/vancomycin composites were coated on hydrothermally etched titanium substrates by casting method. The composite coatings were coated by Melittin through drop casting technique. Melittin raised the proliferation of MC3T3 cells, making it an appropriate option as osteoinductive and antibacterial substance in coatings of orthopedic implants. Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria. Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.

耐甲氧西林和耐万古霉素的金黄色葡萄球菌细菌（分别为 MRSA 和 VRSA）会严重危害骨植入物。本研究旨在检验蜂毒肽和万古霉素在预防 MRSA 和 VRSA 相关骨植入物感染方面的潜在协同作用。壳聚糖/生物活性玻璃纳米粒子/万古霉素复合材料通过浇铸法涂覆在水热蚀刻的钛基材上。复合涂层由Melittin通过滴铸技术涂覆。蜂毒肽提高了 MC3T3 细胞的增殖，使其成为骨科植入物涂层中骨诱导和抗菌物质的合适选择。结合万古霉素和蜂毒肽的复合涂层消除了浮游和粘附的 MRSA 和 VRSA 细菌，而含有其中之一的涂层未能杀死整个 VRSA 细菌。所以，