Anaerobic digestion (AD) is a sustainable technology that combines biogas production with proper waste management. However, the operation of an anaerobic reactor under the stress of nanoparticles (NPs) is unknown. Therefore, this study assessed the disadvantages and advantages of adding two NPs to 11 L semi-continuous stirred-biodigesters and examined their contributions to biogas generation. Iron oxide NPs (INPs) and titanium oxide NPs (TNPs) were mixed daily with manure from dairy cattle and added to the biodigesters daily at concentrations of 100 mg/L (D1) and 500 mg/L (D2), respectively. In addition, a control with no NP supplementation was studied (D0). The results revealed that the generation rates of biogas and CH₄ increased by 25.43 and 62.43%, respectively, for D1 during the first 7-day retention time, as compared with the D0. Conversely, TNP addition reduced the generation rates by 28.76 and 56.92%, respectively, during the same period. Overall, for D1, the biogas and CH₄ yields over the study period increased by 5.48 and 12.35%, respectively, whereas for D2, the rates were reduced by 11.36 and 18.58%, respectively. Therefore, the presence of INPs had a stimulatory influence while that of the TNPs had an inhibitory effect.

厌氧消化 (AD) 是一种可持续技术，将沼气生产与适当的废物管理相结合。然而，在纳米颗粒 (NPs) 应力下厌氧反应器的运行是未知的。因此，本研究评估了向 11 L 半连续搅拌生物消化器中添加两种 NP 的优缺点，并检查了它们对沼气产生的贡献。氧化铁 NPs (INPs) 和氧化钛 NPs (TNPs) 每天与奶牛的粪便混合，每天分别以 100 mg/L (D1) 和 500 mg/L (D2) 的浓度添加到生物消化器中。此外，还研究了不含 NP 补充剂的对照 (D0)。结果表明，沼气和 CH ₄的产生率与 D0 相比，D1 在前 7 天的保留时间内分别增加了 25.43% 和 62.43%。相反，在同一时期，TNP 添加使生成率分别降低了 28.76% 和 56.92%。总体而言，对于 D1，研究期间的沼气和 CH ₄产量分别增加了 5.48% 和 12.35%，而对于 D2，速率分别降低了 11.36% 和 18.58%。因此，INPs 的存在具有刺激作用，而 TNPs 的存在具有抑制作用。