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Microbial sulfite oxidation coupled to nitrate reduction in makeup water for oil production
Chemosphere ( IF 8.8 ) Pub Date : 2021-06-21 , DOI: 10.1016/j.chemosphere.2021.131298
Tekle Tafese Fida 1 , Mohita Sharma 1 , Yin Shen 1 , Gerrit Voordouw 1
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Bisulfite is used as an oxygen scavenger in waters used for oil production to prevent oxygen-mediated pipeline corrosion. Analysis of nitrate-containing water injected with ammonium bisulfite indicated increased concentrations of ammonium, sulfate and nitrite. To understand the microbial process causing these changes, water samples were used in enrichments with bisulfite and nitrate. Oxidation of bisulfite, reduction of nitrate, change in microbial community composition and corrosivity of bisulfite were determined. The results indicated that the microbial community was dominated by Sulfuricurvum, a sulfite-oxidizing nitrate-reducing bacterium (StONRB). Plating of the enriched StONRB culture yielded the bacterial isolate Sulfuricurvum sp. TK005, which coupled bisulfite oxidation with nitrate reduction to form sulfate and nitrite. Bisulfite also induced chemical corrosion of carbon steel at a rate of 0.28 ± 0.18 mm yr−1. Bisulfite and the generated sulfate could serve as electron acceptors for sulfate-reducing microorganisms (SRM), which reduce sulfate and bisulfite to sulfide. Nitrate is frequently injected to injection waters to contain the activity of SRM in oil reservoirs. This study suggests an alternative bisulfite injection procedure: Injection of nitrate after the chemical reaction of bisulfite with oxygen is completed. This could maintain the oxygen scavenger function of bisulfite and SRM inhibitory activity of nitrate.



中文翻译:

用于石油生产的补充水中微生物亚硫酸盐氧化与硝酸盐还原相结合

亚硫酸氢盐在用于石油生产的水中用作除氧剂,以防止氧介导的管道腐蚀。对注入亚硫酸氢铵的含硝酸盐水的分析表明铵、硫酸盐和亚硝酸盐的浓度增加。为了了解导致这些变化的微生物过程,水样被用于富集亚硫酸氢盐和硝酸盐。测定了亚硫酸氢盐的氧化、硝酸盐的还原、微生物群落组成的变化和亚硫酸氢盐的腐蚀性。结果表明,所述微生物群落是由主导Sulfuricurvum,亚硫酸盐氧化的硝酸盐还原菌(StONRB)。富集的 StONRB 培养物的电镀产生了细菌分离株Sulfuricurvum sp TK005,将亚硫酸氢盐氧化与硝酸盐还原结合,形成硫酸盐和亚硝酸盐。亚硫酸氢盐还以 0.28 ± 0.18 mm yr -1的速率引起碳钢的化学腐蚀。亚硫酸氢盐和生成的硫酸盐可以作为硫酸盐还原微生物 (SRM) 的电子受体,将硫酸盐和亚硫酸氢盐还原为硫化物。硝酸盐经常被注入注入水中以控制油藏中 SRM 的活性。该研究提出了一种替代的亚硫酸氢盐注入程序:在亚硫酸氢盐与氧气的化学反应完成后注入硝酸盐。这可以保持亚硫酸氢盐的除氧功能和硝酸盐的 SRM 抑制活性。

更新日期:2021-06-25
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