Purpose of Review

Diabetes mellitus may affect every third adult American by 2050, and about one-third will develop a diabetic foot ulcer (DFU) during their lifetime. The current standard of care results in healing of less than 50% of all DFUs. Many individuals with DFU develop limb-threatening infection which place them at risk for additional morbidity and mortality. We review research associated with culture-independent next-generation sequencing techniques pertaining to diabetic foot ulcers and their potential for clinical application.

Recent Findings

Diabetic foot ulcers are a growing problem and clinicians are limited by their reliance on conventional culture. Metagenomic sequencing technology provides an unparalleled viewpoint of the polymicrobial constituency of DFU. The microbiome techniques used to study the microbial constituency of DFU may offer insight to improve care for these patients, but without standardized approaches in research based on real-world clinical practices, a significant knowledge gap will remain.

中文翻译：

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用于识别糖尿病足溃疡病原体的宏基因组学及其对临床护理的潜在影响

审查目的

到 2050 年，糖尿病可能会影响三分之一的美国成年人，大约三分之一的人在一生中会患上糖尿病足溃疡 (DFU)。当前的护理标准导致所有 DFU 的治愈率低于 50%。许多患有 DFU 的人会发生威胁肢体的感染，这使他们面临额外发病率和死亡率的风险。我们回顾了与不依赖培养的下一代测序技术相关的研究，这些技术与糖尿病足溃疡及其临床应用潜力有关。

最近的发现

糖尿病足溃疡是一个日益严重的问题，临床医生因依赖传统文化而受到限制。宏基因组测序技术为 DFU 的多微生物选区提供了无与伦比的观点。用于研究 DFU 微生物群的微生物组技术可能为改善对这些患者的护理提供见解，但如果没有基于现实世界临床实践的标准化研究方法，将仍然存在重大的知识差距。