Continuous application of Cu-based fungicides and molluscicides may lead to Cu accumulation in vineyard soils. Grapevine (Vitis vinifera L.) inoculation with arbuscular mycorrhizal fungi (AMF) can be a suitable strategy to increase plant tolerance to the excessive levels of this element in the soil. However, the simultaneous use of EDTA-based fertilizers or phytosanitary products in those vineyards can affect metal mobility in the soil, with potential negative consequences for plants. Therefore, this study aimed to elucidate the effect of AMF inoculation on grapevine performance in Cu contaminated soils, in the presence or absence of EDTA chelates.

Two-year-old plants growing in pots filled with an Arenosol, previously inoculated or not with two different AMF, were subjected to three soil treatments: control, Cu addition; Cu and FeNaEDTA addition. Four months later, at the end of the growing season, plant vegetative growth parameters, root colonization as well as soil, root and leaf nutrient contents were analyzed.

In the soil where Cu was applied, Rhizoglomus irregulare- and Funneliformis mosseae-inoculated plants showed significantly higher root biomass than the non-inoculated ones, and R. irregulare-inoculated plants were the ones with the highest root P and Fe concentrations. However, when Cu and FeNaEDTA were applied together to the soil, AMF inoculation was no longer an asset for grapevines, and overall, root biomass decreased, translocation of Cu to leaves was stimulated, and a significant decrease in leaf Mn concentrations was detected, below the levels found in control soils. For this reason, in soils with high Cu-concentrations, although mycorrhizal inoculation can be a suitable strategy to improve plant growth and nutrition, the use of EDTA-based products should be avoided, as it can lead to an amplification of the toxic effects commonly caused by the excess of Cu in plants.

连续使用铜基杀菌剂和杀软体动物剂可能会导致葡萄园土壤中铜的积累。用丛枝菌根真菌 (AMF) 接种葡萄 ( Vitis vinifera L.) 是一种合适的策略，可以提高植物对土壤中这种元素过量水平的耐受性。然而，在这些葡萄园中同时使用基于 EDTA 的肥料或植物检疫产品会影响土壤中金属的流动性，对植物产生潜在的负面影响。因此，本研究旨在阐明在存在或不存在 EDTA 螯合物的情况下，AMF 接种对 Cu 污染土壤中葡萄树性能的影响。

在装有 Arenosol 的盆中生长的两岁植物，之前接种或未接种两种不同的 AMF，进行三种土壤处理：对照、添加铜；添加 Cu 和 FeNaEDTA。四个月后，在生长季节结束时，对植物营养生长参数、根系定植以及土壤、根和叶营养成分进行了分析。

在施用铜的土壤中，接种不规则根霉和毛茛的根系生物量显着高于未接种的植物，不规则根茎植物的根生物量显着高于未接种的植物。- 接种的植物是具有最高根 P 和 Fe 浓度的植物。然而，当 Cu 和 FeNaEDTA 一起施用于土壤时，AMF 接种不再是葡萄树的资产，总体而言，根生物量减少，刺激了 Cu 向叶子的易位，并且检测到叶子 Mn 浓度显着降低，低于在对照土壤中发现的水平。出于这个原因，在高铜浓度的土壤中，虽然菌根接种是改善植物生长和营养的合适策略，但应避免使用基于 EDTA 的产品，因为它通常会导致毒性作用的放大植物体内铜含量过高引起的。