During postharvest life, floret yellowing confines broccoli's economic and nutritional worth. This study aimed to investigate the mechanisms activated by octapeptide NOP-1 (LKRYKRRL-NH₂) treatment at 0, 250, 500, 750, or 1000 μM for retarding floret yellowing in broccoli during storage at 4 °C for 28 d. Our results revealed that the 750 μM NOP-1 treatment retarded chlorophyll degradation by suppressing pheophytinase (PPH) and pheophorbide a oxygenase (PaO) genes expression along with inhibiting Mg²⁺ dechelatase (MDC), and PPH enzymes activity. Besides, 750 μM NOP-1 treatment prevented jasmonic acid (JA) biosynthesis by suppressing phospholipase A (PLA), 13-lipoxygenase (13-LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC), and OPDA reductase (OPR3) genes expression. Interestingly, 750 μM NOP-1 treatment maintained the membrane integrity in broccoli floret, evaluated by malondialdehyde (MDA) accumulation, which could be ascribed to the suppressing phosphatidylinositol 3-kinase (PI3K), phospholipase D (PLD), and 9-lipoxygenase (9-LOX) genes expression. Our results demonstrated that NOP-1, as an innovative, safe, and eco-friendly antisenescence octapeptide, significantly advances our technological proficiency to delay floret yellowing in broccoli during low temperature storage.

在收获后的生活中，小花变黄限制了西兰花的经济和营养价值。本研究旨在研究在 0、250、500、750或 1000 μM的八肽 NOP-1 (LKRYKRRL-NH ₂ ) 处理中激活的机制，以延缓西兰花在4 °C 下储存 28 天期间的小花黄变。我们的结果表明，750 μM NOP-1 处理通过抑制脱镁叶绿素酶 (PPH) 和脱镁叶绿素 a 加氧酶 (PaO) 基因表达以及抑制 Mg ^{2+ 来}延缓叶绿素降解去螯合酶 (MDC) 和 PPH 酶活性。此外，750 μM NOP-1 处理通过抑制磷脂酶 A (PLA)、13-脂肪氧化酶 (13-LOX)、丙二烯氧化合酶 (AOS)、丙二烯氧化环化酶 (AOC) 和 OPDA 还原酶来阻止茉莉酸 (JA) 的生物合成。 OPR3) 基因表达。有趣的是，750 μM NOP-1 处理保持了西兰花小花的膜完整性，通过丙二醛 (MDA) 积累进行评估，这可能归因于抑制磷脂酰肌醇 3-激酶 (PI3K)、磷脂酶 D (PLD) 和 9-脂肪氧化酶 ( 9-LOX) 基因表达。我们的研究结果表明，NOP-1 作为一种创新、安全、环保的抗衰老八肽，显着提高了我们在低温储存过程中延迟西兰花小花变黄的技术水平。