Abstract

Virus and virus-like diseases are major production constraints in vegetatively propagated crops; once a plant is infected, it remains so throughout its life. This work was undertaken to accelerate the process of eliminating apple chlorotic leaf spot virus (ACLSV) from explants obtained from an infected plum tree by in vitro heat therapy combined with meristem tip culture. After a minimum of 1 week of in vitro heat treatment followed by microdissection of meristematic tissues, trees were propagated successfully into virus-free plants. The treatment procedure, from infected plant to virus-free plantlets, takes a minimum of 14 weeks and could be applied to other fruit tree crops. The results of a one-step reverse transcription PCR analysis of treated plants after three accelerated growth cycles indicated the absence of ACLSV. Heat treatment of the test plants, followed by three rounds of indexing, was accomplished in about 28 months, a procedure that could take ≥36 months using conventional methods. The techniques described in this work may help to speed up the treatment of infected plants and screening of planting materials. The application of these procedures will benefit regulatory agencies, allow stakeholders to have timely access to virus-tested material, and facilitate domestic and international trade.

摘要

病毒和病毒样病害是无性繁殖作物的主要生产制约因素；一旦植物被感染，它的一生都会如此。这项工作旨在加速通过体外热疗结合分生组织尖端培养从感染的李树获得的外植体中消除苹果褪绿叶斑病毒 (ACLSV) 的过程。在体外至少 1 周后热处理后对分生组织进行显微切割，树木成功繁殖成无病毒植物。从受感染植物到无病毒幼苗的处理过程至少需要 14 周，并且可以应用于其他果树作物。在三个加速生长周期后对处理过的植物进行一步逆转录 PCR 分析的结果表明不存在 ACLSV。测试植物的热处理，然后是三轮索引，在大约 28 个月内完成，使用传统方法可能需要 ≥36 个月的过程。这项工作中描述的技术可能有助于加快受感染植物的处理和种植材料的筛选。这些程序的应用将使监管机构受益，