ABSTRACT

1. The thymus and bursa of Fabricius are important immune organs in poultry as they play essential roles in sustaining the normal immune function to maintain health. The following trial investigated whether the stocking density affected gene expressions in immune organs.

2. Jinding ducklings were raised in either low or high density (4 or 8 birds/m²) conditions from four to 14 weeks of age, and were then slaughtered and tissues removed. Samples were subjected to high-throughput sequencing to sequence RNA extraction. After filtering calculations with R software, a total of 508 (thymus) and 1,356 (bursa of Fabricius) differentially expressed genes (DEGs) were identified, suggesting that stocking density has an effect on gene expression in duck immune organs.

3. Out of a total of 112 immune factor genes and 112 immune pattern receptor genes in ducks, four thymus and 18 bursa of Fabricius genes were differentially expressed in ducks, which indicated that the change of stocking density could affect the expression of immune genes in poultry.

摘要

1. 胸腺和法氏囊是家禽重要的免疫器官，在维持正常免疫功能以维持健康方面发挥着重要作用。以下试验调查了放养密度是否影响免疫器官中的基因表达。

2. 金顶小鸭在低密度或高密度（4 或 8 只/m ²）条件下饲养4 至 14 周龄，然后屠宰并去除组织。对样品进行高通量测序以对 RNA 提取进行测序。经R软件过滤计算，共鉴定出508个（胸腺）和1356个（法氏囊）差异表达基因（DEGs），表明放养密度对鸭免疫器官基因表达有影响。

3. 鸭共112个免疫因子基因和112个免疫模式受体基因中，有4个胸腺基因和18个法氏囊基因在鸭体内差异表达，说明放养密度的变化会影响鸭群免疫基因的表达。家禽。