ABSTRACT

The natural environment is a primary source of infections caused by members of Burkholderia cepacia complex (BCC), but the release of human waste may in return enrich the natural environment with clinically relevant BCC. Seven BCC isolates from environment influenced by human liquid or solid waste across Croatia, and one clinical isolate was characterised. B. multivorans recovered from the soil at illegal dumpsite belonged to sequence type (ST)19; B. ambifaria from the agricultural soil fertilized with swine or poultry manure to ST927 or new ST; B. cenocepacia from creek sediment, river water and wound swab to new STs. Antimicrobial susceptibility of isolates ranged from sensitive to multidrug-resistant. A variety of bla_TEM genes was confirmed in isolates. Isolates expressed the virulence factors and survived in river water during 50 days. The BCC present natural environments influenced by the human waste are of clinical relevance and a potential source of sporadic infections.

摘要

自然环境是由洋葱伯克霍尔德菌复合体 (BCC) 成员引起的主要感染源，但人类排泄物的释放可能会反过来丰富与临床相关的 BCC 的自然环境。七种 BCC 分离株来自克罗地亚受人类液体或固体废物影响的环境，并鉴定了一种临床分离株。B.从非法垃圾场土壤中回收的多食性动物属于序列类型（ST）19；B.来自用猪粪或家禽粪肥施肥到 ST927 或新 ST 的农业土壤的 ambifaria；B. cenocepacia从小溪沉积物、河水和伤口拭子到新的 ST。分离株的抗菌药物敏感性范围从敏感到多重耐药。各种布拉_TEM基因在分离株中得到证实。分离株表达了毒力因子，并在河水中存活了 50 天。BCC 目前受人类排泄物影响的自然环境具有临床意义，是散发感染的潜在来源。