Abstract

The objective of this study was provide the first report to assess the high-performance liquid chromatography-diode array detection (HPLC-DAD) identification of phytochemicals, the spectrophotometric determination of total phenolics and flavonoids, and antioxidant activities of the root and aerial extracts of Chaerophyllum bulbosum. HPLC-DAD identified the main phytochemical to be rosmarinic acid in the roots in the acetone (CBRA) (13.98 ± 0.12 mg/g) and the aerial water (CBAW) extract; myricetin (8.30 ± 0.22 mg/g) in the root methanol extract (CBRM); trans-aconitic acid (6.46 ± 0.49 mg/g) in the root water extract (CBRW) and fumaric acid in the aerial methanol (CBAM) (17.43 ± 0.87 mg/g) and water CBAW (18.96 ± 0.81 mg/g) extracts. The concentrations of total phenolics and flavonoids were 2.00 ± 0.14-17.79 ± 0.99 µg PEs/mg extract and 2.90 ± 0.00-27.64 ± 0.37 µg QEs/mg extract, respectively. The highest antioxidant activity was found in the root acetone extract (CBRA) using the β-carotene-linoleic (IC₅₀: 172.31 ± 0.16 µg/mL) and CUPRAC (A_0.50: 126.00 ± 0.24 µg/mL) assays and in the root water extract (CBRW) for DPPH^• (IC₅₀: 59.51 ± 0.21 µg/mL) and metal chelating (IC₅₀: 65.91 ± 0.36 µg/mL) assays in the aerial water extract (CBAW) using the ABTS^•+ (IC₅₀: 59.51 ± 0.21 µg/mL) assay. The relationship between the phytochemicals and antioxidant activities of the extracts was chemometrically determined by principal component analysis (PCA) and hierarchical cluster analysis (HCA).

摘要

本研究的目的是提供第一份评估植物化学物质的高效液相色谱-二极管阵列检测 (HPLC-DAD) 鉴定、总酚类和黄酮类化合物的分光光度法测定以及根和空气提取物的抗氧化活性的报告。球茎毛竹。HPLC-DAD 鉴定出丙酮 (CBRA) (13.98 ± 0.12 mg/g) 和气水 (CBAW) 提取物中根中的主要植物化学物质为迷迭香酸；根甲醇提取物 (CBRM) 中的杨梅素 (8.30 ± 0.22 mg/g)；反式-根水提取物 (CBRW) 中的乌头酸 (6.46 ± 0.49 mg/g) 和空中甲醇 (CBAM) (17.43 ± 0.87 mg/g) 和水 CBAW (18.96 ± 0.81 mg/g) 提取物中的富马酸。总酚和类黄酮的浓度分别为 2.00 ± 0.14-17.79 ± 0.99 µg PEs/mg 提取物和 2.90 ± 0.00-27.64 ± 0.37 µg QEs/mg 提取物。使用 β-胡萝卜素-亚油酸 (IC ₅₀ : 172.31 ± 0.16 µg/mL) 和 CUPRAC (A _0.50 : 126.00 ± 0.24 µg/mL) 测定的根丙酮提取物 (CBRA) 和根中的抗氧化活性最高使用 ABTS •+ 在空中水提取物 (CBAW) 中进行 DPPH ^• (IC ₅₀ : 59.51 ± 0.21 µg/mL) 和金属螯合 (IC ₅₀ : 65.91 ± 0.36 µg/mL) 测定的水提取物 (CBRW) ^•+(IC ₅₀ : 59.51 ± 0.21 µg/mL) 测定。提取物的植物化学物质和抗氧化活性之间的关系通过主成分分析（PCA）和层次聚类分析（HCA）进行化学计量学测定。