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Characterization of SHATTERPROOF Homoeologs and CRISPR-Cas9-Mediated Genome Editing Enhances Pod-Shattering Resistance in Brassica napus L.
The CRISPR Journal ( IF 3.7 ) Pub Date : 2021-06-16 , DOI: 10.1089/crispr.2020.0129
Qamar U Zaman 1, 2 , Chu Wen 1 , Shi Yuqin 1 , Hao Mengyu 1 , Mei Desheng 1 , Batley Jacqueline 3 , Zhang Baohong 4 , Li Chao 1 , Hu Qiong 1
Affiliation  

Brassica napus is the most important oil crop plant for edible oil and renewable energy source worldwide. Yield loss caused by pod shattering is a main problem during B. napus harvest. In this study, six BnSHP1 and two BnSHP2 homoeologs were targeted by the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 (CRISPR-associated protein 9) genome editing system and multiple SHP1 and SHP2 mutated lines were identified for evaluating the contribution for pod-shattering resistance. Our data suggest that BnSHP1A09 is probably a promising homoeolog for controlling lignin contents at dehiscence zone. Simultaneous mutation of BnSHP1A09/C04-B/A04 and BnSHP2A05/C04-A exhibited reduced lignified layer and separation layer adjacent to valves and replum. The pod-shattering resistance index (SRI) subsequently increased to 0.31 in five homoeolog mutation lines compared with the wild type (SRI = 0.036), which provide the theoretical basis for breeding of commercial pod-shattering resistance variety.

中文翻译:

SHATTERPROOF 同源物的表征和 CRISPR-Cas9 介导的基因组编辑增强了欧洲油菜的荚果破碎抗性。

欧洲油菜是世界上最重要的食用油和可再生能源油料作物植物。豆荚破碎造成的产量损失是欧洲油菜收获期间的一个主要问题。在这项研究中,CRISPR(成簇规则间隔短回文重复序列)-Cas9(CRISPR 相关蛋白 9)基因组编辑系统靶向了六个BnSHP1和两个BnSHP2同源物,并鉴定了多个SHP1SHP2突变系,用于评估对豆荚的贡献- 抗击破。我们的数据表明BnSHP1A09可能是控制开裂区木质素含量的有前途的同源物。BnSHP1A09/C04-B/A04的同时突变BnSHP2A05/C04-A表现出减少的木质化层和靠近阀门和 relum 的分离层。与野生型(SRI=0.036)相比,5个同源突变系的抗裂荚指数(SRI)随后增加到0.31,为商业抗裂荚品种的选育提供了理论依据。
更新日期:2021-06-21
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