The discovery of CRISPR has revolutionized the field of genome engineering, but the potential of this technology is far from reaching its limits. In this review, we explore the broad range of applications of CRISPR technology to highlight the rapid expansion of the field beyond gene editing alone. It has been demonstrated that CRISPR technology can control gene expression, spatiotemporally image the genome in vivo, and detect specific nucleic acid sequences for diagnostics. In addition, new technologies are under development to improve CRISPR quality controls for gene editing, thereby improving the reliability of these technologies for therapeutics and beyond. These are just some of the many CRISPR tools that have been developed in recent years, and the toolbox continues to diversify.

中文翻译：

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CRISPR 工具箱的多样化：CRISPR-Cas 系统在基因组编辑之外的应用


CRISPR的发现彻底改变了基因组工程领域，但这项技术的潜力还远未达到极限。在这篇综述中，我们探讨了 CRISPR 技术的广泛应用，以强调该领域在基因编辑之外的快速扩展。已经证明，CRISPR技术可以控制基因表达、体内基因组时空成像以及检测特定核酸序列以进行诊断。此外，新技术正在开发中，以改善基因编辑的 CRISPR 质量控制，从而提高这些技术在治疗及其他方面的可靠性。这些只是近年来开发的众多 CRISPR 工具中的一部分，而且工具箱还在不断多样化。