Although asterinaceous fungi have been studied for many years, all previous attempts to isolate, cultivate, and propagate these fungi in vitro have failed. This paper provides the first reports of in vitro isolation of representative strains of species belonging to five fungi from different genera belonging to Asterinales. To confirm if the sequences of DNA obtained from the mycelia are the same obtained in the direct extraction, a phylogenetic analysis of nuc LSU rDNA was performed. This paper reports for the first time the success of in vitro culturing of asterinaceous fungi using the ascospores ejection technique, opening perspectives of studies of genetics, physiology, among other aspects of the biology for this very understudied group of fungi.

尽管对星状真菌进行了多年的研究，但以前在体外分离、培养和繁殖这些真菌的所有尝试都失败了。本文提供了对属于Asterinales不同属的五种真菌的代表性菌株的体外分离的首次报道。为了确认从菌丝体中获得的 DNA 序列是否与直接提取中获得的相同，进行了 nuc LSU rDNA的系统发育分析。这篇论文首次报道了体外成功 使用子囊孢子喷射技术培养星状真菌，为这一极少研究的真菌群开辟了遗传学、生理学以及生物学其他方面的研究前景。