Botrytis cinerea and Penicillium expansum are the most important pathogens of apples during the postharvest phase. In the present study, the effect of ozone (O₃) and carbon dioxide (CO₂) gases combined with application of the yeast isolates Candida membranifaciens A2, Saccharomyces cerevisiae 69, Pichia guilliermondii M47 and A6, was investigated to control blue and gray molds of apples in vitro and in vivo conditions. In vitro, O₃ and CO₂ treatments exerted respectively fungistatic and fungicidal activity on fungal spores. In dual culture tests, the combination of antagonistic yeasts with CO₂ was the most efficient way to control the growth of both pathogens. In vitro, O₃ completely inhibited the growth of yeast isolates, whereas CO₂ did not influence the yeast growth. Inoculation of apples with C. membranifaciens A2, P. guilliermondii A6 or S. cerevisiae PTCC 69 in combination with CO₂ completely suppressed blue or gray mold symptoms for up to 62 days of storage, both at 20 °C and 4 °C. Yeasts combined with O₃ (10 ppm) were poorly effective in controlling disease development on fruit. However, CO₂ or O₃ treatments used alone resulted in a decrease of apple quality after 62 days of storage at 4 °C; necrotic spots were observed for fruits treated with O₃.

Botrytis cinerea和Penicillium expansum是苹果收获后阶段最重要的病原体。在本研究中，臭氧 (O ₃ ) 和二氧化碳 (CO ₂ ) 气体与酵母分离株Candida meembranifaciens A2、Saccharomyces cerevisiae 69、Pichia guilliermondii M47 和 A6 的应用相结合，对控制蓝色和灰色霉菌的效果进行了研究苹果的体外和体内条件。在体外，O ₃和CO ₂处理分别对真菌孢子发挥抑菌和杀真菌活性。在双重培养试验中，拮抗酵母与 CO ₂的组合是控制两种病原体生长的最有效方法。在体外，O ₃完全抑制酵母分离株的生长，而CO ₂不影响酵母的生长。用苹果的接种C. membranifaciens A2，P.季也蒙A6或酿酒酵母PTCC 69结合CO ₂完全抑制蓝色或灰色模具症状多达62天的储存的，无论是在20℃和4℃。酵母与 O ₃ (10 ppm)结合在控制果实病害发展方面效果不佳。然而，CO ₂或 O ₃在 4°C 下储存 62 天后，单独使用的处理导致苹果品质下降；用O ₃处理的果实观察到坏死斑点。