The Puma lineage within the family Felidae consists of 3 species that last shared a common ancestor around 4.9 million years ago. Whole-genome sequences of 2 species from the lineage were previously reported: the cheetah (Acinonyx jubatus) and the mountain lion (Puma concolor). The present report describes a whole-genome assembly of the remaining species, the jaguarundi (Puma yagouaroundi). We sequenced the genome of a male jaguarundi with 10X Genomics linked reads and assembled the whole-genome sequence. The assembled genome contains a series of scaffolds that reach the length of chromosome arms and is similar in scaffold contiguity to the genome assemblies of cheetah and puma, with a contig N50 = 100.2 kbp and a scaffold N50 = 49.27 Mbp. We assessed the assembled sequence of the jaguarundi genome using BUSCO, aligned reads of the sequenced individual and another published female jaguarundi to the assembled genome, annotated protein-coding genes, repeats, genomic variants and their effects with respect to the protein-coding genes, and analyzed differences of the 2 jaguarundis from the reference mitochondrial genome. The jaguarundi genome assembly and its annotation were compared in quality, variants, and features to the previously reported genome assemblies of puma and cheetah. Computational analyzes used in the study were implemented in transparent and reproducible way to allow their further reuse and modification.

中文翻译：

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难以捉摸的 Jaguarundi，Puma yagouaroundi 的从头基因组组装草案

猫科动物家族中的美洲狮血统由 3 个物种组成，它们最后共享一个共同祖先大约是在 490 万年前。先前报道了来自该谱系的 2 个物种的全基因组序列：猎豹 (Acinonyx jubatus) 和美洲狮 (Puma concolor)。本报告描述了其余物种的全基因组组装，美洲虎（美洲狮 yagouaroundi）。我们使用 10X Genomics 关联读数对雄性美洲虎的基因组进行了测序，并组装了全基因组序列。组装的基因组包含一系列达到染色体臂长度的支架，并且支架邻接性与猎豹和美洲狮的基因组组装相似，contig N50 = 100.2 kbp 和支架 N50 = 49.27 Mbp。我们使用 BUSCO 评估了美洲虎基因组的组装序列，将测序个体和另一只已发表的雌性美洲虎与组装基因组的读数对齐，注释蛋白质编码基因、重复序列、基因组变异及其对蛋白质编码基因的影响，并分析 2 只美洲虎与参考线粒体基因组的差异. 美洲虎基因组组装及其注释在质量、变异和特征上与先前报道的美洲狮和猎豹基因组组装进行了比较。研究中使用的计算分析以透明和可重复的方式实施，以便进一步重用和修改。并分析了 2 只美洲虎与参考线粒体基因组的差异。美洲虎基因组组装及其注释在质量、变异和特征上与先前报道的美洲狮和猎豹基因组组装进行了比较。研究中使用的计算分析以透明和可重复的方式实施，以便进一步重用和修改。并分析了 2 只美洲虎与参考线粒体基因组的差异。美洲虎基因组组装及其注释在质量、变异和特征上与先前报道的美洲狮和猎豹基因组组装进行了比较。研究中使用的计算分析以透明和可重复的方式实施，以便进一步重用和修改。