The anaerobic digestion (AD) of organic fractions of municipal solid waste (OFMSWs) represents a promising solution for achieving greater landfill diversion and resource recycling. The objectives of this study were (1) to explore potential synergistic effects on methane production of co-digestion of organic MSW components with distinct levels of biodegradability and (2) to examine whether and how the inoculum source affect those synergistic effects on methane yields and kinetics. Anaerobic mono- and co-digestion of food waste (FW), newsprint paper (NP), and branches (BR) were conducted in a batch culture system inoculated with landfill leachate and anaerobic sludge under mesophilic conditions. The methane generation results showed that co-digestion of readily degradable FW with more recalcitrant lignocellulosic NP and/or BR resulted in additive effects on cumulative yield but synergistic effects on production rates (up to 22% yield increase between 10 and 15 days). This early synergism was primarily associated with the accelerated hydrolysis due to the addition of FW that promoted the growth of hydrolytic microorganisms. A 16S rRNA sequencing-based community analysis revealed that the microbial communities were primarily influenced by their inoculum and cannot explain for the observed synergy. These results suggest that when multi-component municipal waste is used as AD feedstock, the early synergistic benefits can be considered for process design and optimization.

城市固体废物 (OFMSW) 有机部分的厌氧消化 (AD) 代表了实现更大的垃圾填埋场转移和资源回收的有前景的解决方案。本研究的目的是 (1) 探索具有不同生物降解性水平的有机 MSW 组分共同消化对甲烷产生的潜在协同效应，以及 (2) 检查接种源是否以及如何影响这些协同效应对甲烷产量和动力学。在中温条件下，在接种垃圾渗滤液和厌氧污泥的分批培养系统中对食物垃圾 (FW)、新闻纸 (NP) 和树枝 (BR) 进行厌氧单消化和共消化。甲烷生成结果表明，易降解的 FW 与更顽固的木质纤维素 NP 和/或 BR 的共同消化对累积产量产生累加效应，但对生产率产生协同效应（10 到 15 天之间产量增加高达 22%）。由于添加促进水解微生物生长的 FW，这种早期协同作用主要与加速水解有关。基于 16S rRNA 测序的群落分析显示，微生物群落主要受其接种物的影响，无法解释观察到的协同作用。这些结果表明，当多组分城市垃圾用作 AD 原料时，可以考虑早期协同效益进行工艺设计和优化。