ABSTRACT

Nickel oxide nanoparticles (NiO NPs) were synthesized via the facile sol–gel method. The synthesized NiO NPs were characterized using X-ray diffraction (XRD), Field-Emission Scanning Electron Microscopy (FESEM), Transmission Electron Microscopy (TEM), Raman and Fourier Transform Infrared (FT-IR) techniques. The effect of particle size was analyzed on structural alterations, electrochemical behaviors, and cytotoxic effect of synthesized NiO NPs. According to TEM results, the particle sizes of synthesized NiO NPs were 8.2, 15.4, and 21.7 nm at 300, 400, and 500 °C, respectively. Electrochemical behaviors of synthesized NiO NPs were assessed through the cyclic voltammetry (CV) technique. The results showed that the magnitude of the current density was decreased in treated samples exposed to higher temperature values. The cytotoxic activity of synthesized nanoparticles was investigated against human liver cancer cell (Hep-G2), breast cancer cell (MCF-7), and colon cancer cell (HT-29) lines using the MTT assay. The results demonstrated that synthesized NiO NPs had higher cytotoxicity at 300 ˚C than at 400 and 500 ˚C, because of their small particle size. Thus, synthesized NiO NPs exhibit acceptable cytotoxic effects against Hep-G2, MCF-7, and HT-29 cancer cell lines so that they could be a good choice for cancer treatment.

摘要

氧化镍纳米颗粒（NiO NPs）是通过简便的溶胶-凝胶法合成的。使用 X 射线衍射 (XRD)、场发射扫描电子显微镜 (FESEM)、透射电子显微镜 (TEM)、拉曼和傅里叶变换红外 (FT-IR) 技术对合成的 NiO NP 进行表征。分析了粒径对合成的 NiO NPs 的结构改变、电化学行为和细胞毒性作用的影响。根据 TEM 结果，合成的 NiO NPs 在 300、400 和 500 °C 下的粒径分别为 8.2、15.4 和 21.7 nm。通过循环伏安法 (CV) 技术评估合成的 NiO NPs 的电化学行为。结果表明，在暴露于较高温度值的处理样品中，电流密度的大小降低。使用 MTT 测定法研究合成纳米颗粒对人肝癌细胞 (Hep-G2)、乳腺癌细胞 (MCF-7) 和结肠癌细胞 (HT-29) 系的细胞毒活性。结果表明，合成的 NiO NPs 在 300 ˚C 下具有比 400 和 500 ˚C 更高的细胞毒性，因为它们的粒径小。因此，合成的 NiO NPs 对 Hep-G2、MCF-7 和 HT-29 癌细胞系表现出可接受的细胞毒性作用，因此它们可能是癌症治疗的不错选择。