Ascorbic acid (AA) is one of the vital water-soluble antioxidants in blood serum and can function as a key blood serum biomarker for oxidative stress. Therefore, we have developed a highly sensitive and selective AA electrochemical sensor based on novel porous silicon-mesoporous carbon nanocomposites (PSi-MC NCs) modified glassy carbon electrode (GCE). The structural properties and morphological investigation of the PSi-MC NCs were systematically studied by different techniques. This newly designed PSi-MC/GCE-based non-enzymatic electrochemical sensor can measure an extremely wide range of AA (0.5–2473 μM) in phosphate buffer solution (PBS) with a sensitivity 0.1982 μAμM⁻¹cm⁻² and a logical limit of detection (LOD) 30.0±0.1 nM. The proposed AA sensor was also applied to detect possible interference from common interfering substances that are present in human blood, showing highly selective performance. The proposed AA sensor also showed highly acceptable results towards the detection of AA levels in human blood serum and vitamin C tablets. This new combination of active material PSi-MC NCs provided steady, excellent reproducibility, repeatability, and long-term stability.

抗坏血酸 (AA) 是血清中重要的水溶性抗氧化剂之一，可以作为氧化应激的关键血清生物标志物。因此，我们开发了一种基于新型多孔硅-介孔碳纳米复合材料 (PSi-MC NCs) 修饰的玻碳电极 (GCE) 的高灵敏度和选择性 AA 电化学传感器。通过不同的技术系统地研究了 PSi-MC NCs 的结构特性和形态学研究。这种新设计的基于 PSi-MC/GCE 的非酶电化学传感器可以测量磷酸盐缓冲溶液 (PBS) 中极宽范围的 AA (0.5–2473 μM)，灵敏度为 0.1982 μAμM ^-1 cm ^-2和逻辑检测限 (LOD) 30.0±0.1 nM。提议的 AA 传感器还用于检测人体血液中常见干扰物质的可能干扰，显示出高度选择性的性能。提议的 AA 传感器在检测人血清和维生素 C 片剂中的 AA 水平方面也显示出高度可接受的结果。这种活性材料 PSi-MC NC 的新组合提供了稳定、出色的重现性、可重复性和长期稳定性。