In recent times, carbamazepine (CBZ) as an anticonvulsants drug has raised attention because of its safety concern in the aquatic environment. The present study aimed to evaluate the sub-lethal effects of CBZ (1%, 0.1 % and 0.01 % of 96 h LC₅₀) on P. hypophthalmus for 60 days based on haematological, biochemical, and genotoxicity biomarkers. Chronic exposure of CBZ altered blood profiles (total erythrocyte count, packed cell volume, haemoglobin) and serum biomarkers such as alkaline phosphates, cholesterol, lactate dehydrogenase and transaminase enzymes. Oxidative stress biomarkers such as superoxide dismutase (SOD) and catalase (CAT) activity were also substantially affected in all treatments. Genotoxicity study revealed the formation of micronucleus in erythrocytes of exposed fish. Integrated Biomarker Response (IBR) study showed cholesterol, serum glutamic oxaloacetic transaminase (SGOT) in serum and SOD, CAT in liver tissue are the best organ-based enzyme biomarkers. The present report concludes that an environmentally realistic concentration of CBZ can pose a serious threat to aquatic organisms.

近年来，卡马西平 (CBZ) 作为一种抗惊厥药物因其在水生环境中的安全问题而受到关注。本研究旨在评估 CBZ（96 小时 LC _{50 的}1%、0.1% 和 0.01% ）对下眼底假单胞菌的亚致死作用基于血液学、生化和遗传毒性生物标志物，持续 60 天。CBZ 的慢性暴露会改变血液特征（总红细胞计数、压实细胞体积、血红蛋白）和血清生物标志物，如碱性磷酸盐、胆固醇、乳酸脱氢酶和转氨酶。在所有治疗中，氧化应激生物标志物如超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 活性也受到显着影响。基因毒性研究揭示了在暴露的鱼的红细胞中形成了微核。综合生物标志物反应 (IBR) 研究表明，血清中的胆固醇、血清谷氨酸草酰乙酸转氨酶 (SGOT) 和肝组织中的 SOD、CAT 是最好的基于器官的酶生物标志物。本报告得出结论，CBZ 的环境现实浓度会对水生生物构成严重威胁。